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Eurosurveillance, Volume 5, Issue 4, 01 April 2000
Articles
Feasibility of surveillance resistance to antituberculosis drugs :Europe, 1997

Citation style for this article: Schwoebel V, Antoine D, Veen J. Feasibility of surveillance resistance to antituberculosis drugs :Europe, 1997 . Euro Surveill. 2000;5(4):pii=36. Available online: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=36

V. Schwœbel1, D. Antoine1, J. Veen2 and the national coordinators for tuberculosis surveillance in Denmark, Estonia, Finland, Iceland, Netherlands, Norway, Romania, Slovenia, Sweden and Switzerland*
1 Institut de Veille Sanitaire, Saint-Maurice, France
2 Royal Netherlands Tuberculosis Association, The Hague, The Netherlands
*Denmark: Else Smith; Estonia: Annika Kruuner, Kai Vink; Finland: Petri Ruutu; Iceland: Thorsteinn Blöndal, Netherlands: Jaap Veen; Norway: Einar Heldal; Romania: Emil Corlan; Slovenia: Jurij Sorli; Sweden: Victoria Romanus: Switzerland: Peter Helbling.


Between 28% and 100% of isolates from ten countries from the EuroTB network in 1997 were reported with data on antituberculosis drug susceptibility. Drug resistance was commoner among foreign-born patients and in general among patients who had been treated previously for tuberculosis.

The EuroTB programme has been collecting, analysing, and disseminating epidemiological information on tuberculosis in the European Union and in the other countries of the World Health Organization (WHO) European Region since 1996 (1,2). The feasibility of monitoring resistance to antituberculosis drugs was studied in 1997 among countries that take part in EuroTB and provide individual computerised data.

Methods

Recommendations on the standardisation of the surveillance of resistance to antituberculosis drugs in Europe elaborated by a working group of WHO and the International Union Against Tuberculosis and Lung Disease were approved recently by European country representatives (3). Based on these recommendations, national representatives were asked to provide data on susceptibility to isoniazid, rifampicin, ethambutol, and streptomycin at the start of treatment for all culture positive cases of tuberculosis notified in 1997. Only results reported nationally and provided as data related to individual cases in the computerised data file for tuberculosis notifications were accepted.

Drug susceptibility results were analysed by age, geographic origin of the patient (based on country of birth if available, or on citizenship), and history of previous treatment (defined by one month or more of curative treatment by combined antituberculosis drugs or, if information on treatment was unavailable, by a previous diagnosis of active tuberculosis).

Results

Ten of the 19 countries that provided computerised data on individual cases of tuberculosis notified in 1997 supplied results of drug susceptibility testing at the start of treatment: Denmark, Estonia, Finland, Iceland, the Netherlands, Norway, Romania, Slovenia, Sweden, and Switzerland (table).

Table. Drug resistance at start of treatment among culture-positive tuberculosis cases. 1997 (10 countries)

Country

Culture(+)

Tested

Isoniazid resistant

Rifampicin resistant

Multi-drug resistance

Ethambutol resistant

Streptomycin resistant

N

N

N

(%)

N

(%)

N

(%)

N

(%)

N

(%)

Denmark*

459

425

20

(4.7)

3

(0.7)

1

(0.2)

2

(0.5)

44

(10.4)

Estonia

595

378

88

(23.3)

52

(13.8)

49

(13.0)

28

(7.4)

97

(25.7)

Finland*

445

320

14

(4.4)

2

(0.6)

1

(0.3)

0

(0.0)

4

(1.3)

Iceland

9

9

0

(0.0)

0

(0.0)

0

(0.0)

0

(0.0)

-

-

Netherlands

905

905

59

(6.5)

11

(1.2)

7

(0.8)

3

(0.3)

68

(7.5)

Norway

139

133

11

(8.3)

1

(0.8)

1

(0.8)

0

(0.0)

-

-

Romania

13 726

3822

402

(10.5)

246

(6.4)

131

(3.4)

42

(1.1)

703

(18.4)

Slovenia

356

315

5

(1.6)

3

(1.0)

3

(1.0)

2

(0.6)

-

-

Sweden

380

380

24

(6.3)

4

(1.1)

4

(1.1)

0

(0.0)

18

(4.7)

Switzerland

585

453

23

(5.1)

6

(1.3)

5

(1.1)

4

(0.9)

-

-

Patients never treated** (9 countries)

Country

Culture(+)

Tested

Isoniazid resistant

Rifampicin resistant

Multi-drug resistance

Ethambutol resistant

Streptomycin resistant

N

N

N

(%)

N

(%)

N

(%)

N

(%)

N

(%)

Denmark*

421

391

20

(5.1)

2

(0.5)

1

(0.3)

2

(0.5)

42

(10.7)

Estonia

520

334

76

(22.8)

46

(13.8)

43

(12.9)

21

(6.3)

83

(24.9)

Iceland

7

7

0

(0.0)

0

(0.0)

0

(0.0)

0

(0.0)

-

-

Netherlands

844

844

51

(6.0)

9

(1.1)

5

(0.6)

1

(0.1)

60

(7.1)

Norway

135

129

11

(8.5)

1

(0.8)

1

(0.8)

0

(0.0)

-

-

Romania

12 132

3323

316

(9.5)

188

(5.7)

94

(2.8)

33

(1.0)

600

(18.1)

Slovenia

316

280

3

(1.1)

2

(0.7)

2

(0.7)

2

(0.7)

-

-

Sweden

354

354

20

(5.6)

2

(0.6)

2

(0.6)

0

(0.0)

17

(4.8)

Switzerland

437

345

11

(3.2)

0

(0.0)

0

(0.0)

0

(0.0)

-

-

Patients previously treated ** (9 countries)

Country

Culture(+)

Tested

Isoniazid resistant

Rifampicin resistant

Multi-drug resistance

Ethambutol resistant

Streptomycin resistant

N

N

N

(%)

N

(%)

N

(%)

N

(%)

N

(%)

Denmark*

38

34

0

(0.0)

1

(2.9)

0

(0.0)

0

(0.0)

2

(5.9)

Estonia

75

44

12

(27.3)

6

(13.6)

6

(13.6)

7

(15.9)

14

(31.8)

Iceland

2

2

0

(0.0)

0

(0.0)

0

(0.0)

0

(0.0)

-

-

Netherlands

61

61

8

(13.1)

2

(3.3)

2

(3.3)

2

(3.3)

8

(13.1)

Norway

4

4

0

(0.0)

0

(0.0)

0

(0.0)

0

(0.0)

-

-

Romania

1594

499

86

(17.2)

58

(11.6)

37

(7.4)

9

(1.8)

103

(20.6)

Slovenia

39

34

2

(5.9)

1

(2.9)

1

(2.9)

0

(0.0)

-

-

Sweden

26

26

4

(15.4)

2

(7.7)

2

(7.7)

0

(0.0)

1

(3.8)

Switzerland

53

42

10

(23.8)

6

(14.3)

5

(11.9)

4

(9.5)

-

-

* Provisional data which are subject to revision
** By ³ 1 month of curative treatment with combined antituberculosis drugs
In countries where information on previous treatment is not reported. a previous tuberculosis diagnosis is considered as a previous treatment)

Streptomycin was not tested systematically with other drugs in Iceland, Norway, Slovenia, and Switzerland, so results of streptomycin susceptibility are not presented for these four countries. All culture positive cases were supplied with results for isoniazid, rifampicin, and ethambutol susceptibility by Iceland, the Netherlands, and Sweden, compared with 96% of culture positive cases by Norway, 93% by Denmark, 88% by Slovenia, 77% by Switzerland, 72% by Finland, 64% by Estonia, and 28% by Romania.

Proportions of isolates with drug resistance varied widely by country. Two groups were defined according to the proportion of isolates with multidrug resistance (MDR) among all culture positive patients:

  • in eight countries (Denmark, Finland, Iceland, Netherlands, Norway, Slovenia, Sweden, and Switzerland) MDR accounted for 0% (Iceland) to 1.1% (Sweden and Switzerland);
  • in Romania and Estonia MDR accounted for 3.4% and 13.0%, respectively.

In the first group of countries, higher proportions of patients of foreign origin had resistant isolates than nationals. The proportions of isolates with isoniazid resistance were higher in patients from Asia (9.2%), Africa (9.6%), or foreign European countries (3.7%), than in nationals (2.3%), as was observed for streptomycin resistance (10.1%, 12.9%, 2.8%, and 2.0%, respectively) and for MDR (1.3%, 1.3%, 0.6%, and 0.1%, respectively (figure). These differences by geographic origin were seen in all countries apart from Slovenia and were found both in patients who had and who had not been treated previously.

All countries but Finland reported whether patients had been treated for or diagnosed with tuberculosis previously. Among the nine countries, the proportions of drug resistant isolates were higher among patients previously treated than among those never treated (table). There were a few exceptions, however: the proportions of resistant isolates were higher in patients never treated than in patients previously treated in Denmark (for all drugs), in Sweden (for streptomycin), and in Norway (for isoniazid and rifampicin). This was attributable to the large proportions of foreign-born patients (70% in Denmark, 68% in Sweden, 53% in Norway) among culture positive patients never previously treated, in whom the proportions of resistant isolates were much higher than in nationals.

Among patients never treated and originating from the country of diagnosis, the proportions of isolates resistant to isoniazid and rifampicin were lower in younger (<35 years) than in older patients. Exceptions were Romania and Estonia, however, where high proportions of drug resistant isolates, and particularly high proportions of isolates resistant to rifampicin and of MDR, were observed in younger patients (higher than in older patients in Estonia).

Discussion

These data provide a preliminary sketch of antituberculosis drug resistance in Europe. Results are geographically limited, however, and should not be taken as definitive: since the feasibility study, additional data from the same period have been added in some countries (Denmark, Finland). Comparisons between countries should be made with caution because of differences in the proportions of tuberculosis cases for whom results were available (this proportion was particularly low in Romania). In addition, comparisons are difficult to make since they are often based on very small numbers, particularly among patients previously treated.

Since antituberculosis drug resistance results from the selection of drug resistant mutant bacilli following inadequate use of drugs (4), high proportions of drug resistant isolates reflect present and past misuse of antituberculosis drugs. The high proportions of isolates with resistance observed in Estonia and Romania may reflect problems in treatment delivery, as occurred in some republics of the former Soviet Union (5). Furthermore, the high proportions of drug resistant isolates among young patients who had not been treated before suggest that transmission of drug resistant bacilli has been occurring recently in the community or in some institutions.

In contrast, the relatively low levels of drug resistance found in the five Scandinavian countries, the Netherlands, Slovenia, and Switzerland, and particularly the low proportions of resistance among nationals, probably indicate a good quality of tuberculosis treatment. In all of these countries except Slovenia, the epidemiology of drug resistance appears to be influenced largely by patients of foreign origin, who made up a large proportion of cases with drug resistant infections. Patients originating from some countries (such as in Asia or Africa) may be at high risk of infection with drug resistant bacilli as well as for acquiring drug resistance during treatment in their countries of origin. There may also be specific problems in case management and/or difficult living conditions, however, which expose immigrants to the risk of both primary and acquired drug resistance in the country of diagnosis.

These results show that it is feasible to integrate the surveillance of drug resistance within the tuberculosis notification system. The linkage of drug susceptibility results with data of the notification allows the representativeness of data to be assessed and the relevant information (particularly age, previous history of tuberculosis treatment, and patient’s geographic origin) to be analysed. EuroTB is planning to increase its collection of data on drug resistance in future years.

EuroTB is funded by the Directorate General Health and Consumer Protection (DG SANCO/F4) of the Commission of the European Communities.


References

1. Perrocheau A, Schwoebel V, Veen J, and the National Coordinators for Tuberculosis Surveillance in 46 Countries of the WHO European Region. Surveillance of tuberculosis in the WHO European Region in 1995: results of the feasibility study. Eurosurveillance 1998; 3: 2-5.

2. Antoine D, Schwoebel V, Veen J, Raviglione M, Rieder HL, and the National Coordinators for Tuberculosis Surveillance in 50 Countries of the WHO European Region. Surveillance of tuberculosis in the WHO European Region, 1995-1996. Eurosurveillance 1998; 3: 103-7.

3. Schwœbel V, Lambregts-van Weezenbeek CSB, Moro ML, Drobniewski F, Hoffner SE, Raviglione MC, et al. Standardisation of antituberculosis drug resistance surveillance in Europe. Recommendations of a Working Group of the World Health Organization (WHO) and the European Region of the International Union Against Tuberculosis and Lung Disease (IUATLD). Eur Respir J 2000 (in press).

4. Canetti G. The J. Burns Amberson Lecture. Present aspects of bacterial resistance in tuberculosis. Am Rev Respir Dis 1965; 92: 687-703.

5. Zalesky R, Abdullajev F, Khechinachvili G, Safarian M, Madaras T, Grzemska M, et al. Tuberculosis control in the Caucasus: successes and contraints in DOTS implementation. Int J Tuberc Lung Dis 1999; 3: 394-401.

 



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