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Abstract

We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.

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/content/10.2807/1560-7917.ES2013.18.14.20441
2013-04-04
2017-10-20
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES2013.18.14.20441
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