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2025 theme: Vaccines

Abstract

We report a case of a mild, self-limited rash illness in a child 18 days after measles-mumps-rubella-varicella vaccination. Initial testing with a PCR-based method failed to detect vaccine virus. Sequencing later identified a novel mutation in the probe-binding site of the vaccine assay that had arisen after vaccination and resulted in the false-negative PCR test results.

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/content/10.2807/1560-7917.ES.2025.30.43.2500774
2025-10-30
2025-11-12
/content/10.2807/1560-7917.ES.2025.30.43.2500774
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Measles vaccine virus mutation following vaccination in a healthy child resulting in a false negative vaccine specific PCR test: Ontario, Canada, 2025
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Sarah+E+Wilson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wilson Sarah E</a>, <a href="/search?value1=Vanessa+Zubach&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Zubach Vanessa</a>, <a href="/search?value1=Brendan+Lew&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lew Brendan</a>, <a href="/search?value1=Maan+Hasso&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hasso Maan</a>, <a href="/search?value1=Romy+Olsha&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Olsha Romy</a>, <a href="/search?value1=Marina+I+Salvadori&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Salvadori Marina I</a>, <a href="/search?value1=Navya+Manoj&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Manoj Navya</a>, <a href="/search?value1=Joanne+Hiebert&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hiebert Joanne</a></span>. Measles vaccine virus mutation following vaccination in a healthy child resulting in a false negative vaccine specific PCR test: Ontario, Canada, 2025. <a href="/content/ecdc">Euro Surveill.</a> 2025;30(43):pii=2500774. <a href="https://doi.org/10.2807/1560-7917.ES.2025.30.43.2500774" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2025.30.43.2500774</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 07 Oct 2025;&nbsp;&nbsp; <span class="generated">Accepted</span>: 28 Oct 2025 </span> </p>
/content/10.2807/1560-7917.ES.2025.30.43.2500774
/content/10.2807/1560-7917.ES.2025.30.43.2500774
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