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Abstract

BACKGROUND

In the Netherlands, locally acquired animal and human cases of West Nile virus (WNV) were first identified in 2020 via multidisciplinary WNV monitoring and research activities focusing on mosquitoes, birds, horses, and humans.

AIM

We investigated how different activities contributed to WNV detection and response in the Netherlands between 2020 and 2023, to determine best practices, challenges, and opportunities for improvement.

METHODS

We identified WNV monitoring and research activities in the Netherlands from 2020 to 2023 and analysed their timeliness to detect and react to WNV circulation. An after-action review (AAR) was conducted with national WNV experts to assess best practices and challenges in the multidisciplinary approach.

RESULTS

In 2020, WNV circulation was discovered in an infected bird through a wild live bird research survey and subsequently through mosquito research and monitoring. Thirty-five days after finding the WNV-PCR-positive bird, the first autochthonous human case was uncovered. Between 2021 and 2023, research projects in animals, including sentinel chickens, detected ongoing local enzootic WNV circulation. The AAR highlighted rapid information sharing and interpretation, enabled by multidisciplinary collaborations, as best practice. However, differing institute priorities could sometimes lead to diverging views on follow-up actions.

CONCLUSION

Research and monitoring activities in mosquitoes and animals, particularly wild birds and sentinel chickens, enabled early detection of WNV circulation. Real-time testing could provide early warning of human cases, enabling timely responses. Therefore, these research and monitoring activities should be maintained. Multidisciplinary collaboration enabled rapid detection and response, and addressing remaining challenges could further strengthen effectiveness.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2026-03-12
2026-04-19
/content/10.2807/1560-7917.ES.2026.31.10.2500276
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References

  1. Lu L, Zhang F, Oude Munnink BB, Munger E, Sikkema RS, Pappa S, et al. West Nile virus spread in Europe: Phylogeographic pattern analysis and key drivers. PLoS Pathog. 2024;20(1):e1011880.  https://doi.org/10.1371/journal.ppat.1011880  PMID: 38271294 
  2. Colpitts TM, Conway MJ, Montgomery RR, Fikrig E. West Nile Virus: biology, transmission, and human infection. Clin Microbiol Rev. 2012;25(4):635-48.  https://doi.org/10.1128/CMR.00045-12  PMID: 23034323 
  3. Bakonyi T, Ferenczi E, Erdélyi K, Kutasi O, Csörgő T, Seidel B, et al. Explosive spread of a neuroinvasive lineage 2 West Nile virus in Central Europe, 2008/2009. Vet Microbiol. 2013;165(1-2):61-70.  https://doi.org/10.1016/j.vetmic.2013.03.005  PMID: 23570864 
  4. Lustig Y, Sofer D, Bucris ED, Mendelson E. Surveillance and diagnosis of west nile virus in the face of flavivirus cross-reactivity. Front Microbiol. 2018;9:2421.  https://doi.org/10.3389/fmicb.2018.02421  PMID: 30369916 
  5. Bakonyi T, Haussig JM. West Nile virus keeps on moving up in Europe. Euro Surveill. 2020;25(46):2001938.  https://doi.org/10.2807/1560-7917.ES.2020.25.46.2001938  PMID: 33213684 
  6. European Centre for Disease Prevention and Control (ECDC)European Food Safety Authority (EFSA). Surveillance of West Nile virus infections in humans and animals in Europe, monthly report. EFSA J. 2025;23(7):e9594.  https://doi.org/10.2903/j.efsa.2025.9594  PMID: 40655556 
  7. Gossner CM, Marrama L, Carson M, Allerberger F, Calistri P, Dilaveris D, et al. West Nile virus surveillance in Europe: moving towards an integrated animal-human-vector approach. Euro Surveill. 2017;22(18):30526.  https://doi.org/10.2807/1560-7917.ES.2017.22.18.30526  PMID: 28494844 
  8. Braks MAH, van den Kerkhof JHTC. Westnijlvirus in Nederland, Aanpak surveillance en reponse 2021-2023. Bilthoven: National Institute for Public Health and the Environment; 2021.
  9. One Health PACT. Available from: https://www.onehealthpact.org/.
  10. European Centre for Disease Prevention and Control (EDCD). West Nile virus risk assessment tool. Stockholm: ECDC; 2013. Available from: www.ecdc.europa.eu
  11. World Health Organisation (WHO). Guidance for After Action Review (AAR). Geneva: WHO; 2019.
  12. Sikkema RS, Schrama M, van den Berg T, Morren J, Munger E, Krol L, et al. Detection of West Nile virus in a common whitethroat (Curruca communis) and Culex mosquitoes in the Netherlands, 2020. Euro Surveill. 2020;25(40):2001704.  https://doi.org/10.2807/1560-7917.ES.2020.25.40.2001704  PMID: 33034280 
  13. Vlaskamp DRM, Thijsen SFT, Reimerink J, Hilkens P, Bouvy WH, Bantjes SE, et al. First autochthonous human West Nile virus infections in the Netherlands, July to August 2020. Euro Surveill. 2020;25(46):1-4.  https://doi.org/10.2807/1560-7917.ES.2020.25.46.2001904  PMID: 33213687 
  14. Taks NJLM, Bouvy WH, Thijsen SFT, Reimerink JHJ, Reuskens CBEM, Raven CFH. Meningitis op basis van een autochtone infectie met westnijlvirus. [Meningitis due to an autochthonous infection with West Nile virus]. Tijdschr Neurol Neurochir. 2021;122(4):175-9.
  15. Reimerink JHJ, Braks MAH, Voordouw BCG, van den Wijngaard CC, van den Kerkhof HCT, Reusken CBEM. Westnijlvirus: virologie, epidemiologie, klinische beelden en diagnostiek. [West Nile virus: virology, epidemiology, clinical manifestations, and diagnostics]. Tijdschrift Infectieziekten.2021;16(4):126-32.
  16. van der Giessen J, Vlaanderen F, Kortbeek T, Swaan C, van den Kerkhof H, Broens E, et al. Signalling and responding to zoonotic threats using a One Health approach: a decade of the Zoonoses Structure in the Netherlands, 2011 to 2021. Euro Surveill. 2022;27(31):2200039.  https://doi.org/10.2807/1560-7917.ES.2022.27.31.2200039  PMID: 35929428 
  17. Braks MAH, Duijster JW, Stroo CAJ. Het westnijlvirus in 2020 toch in Nederland. [West Nile virus unexpectedly in the Netherlands]. Ned Tijdschr Geneeskd. 2022;166:D6278. PMID: 35499516 
  18. de Bellegarde de Saint Lary C, Kasbergen LMR, Bruijning-Verhagen PCJL, van der Jeugd H, Chandler F, Hogema BM, et al. Assessing West Nile virus (WNV) and Usutu virus (USUV) exposure in bird ringers in the Netherlands: a high-risk group for WNV and USUV infection? One Health. 2023;16:100533.  https://doi.org/10.1016/j.onehlt.2023.100533  PMID: 37363259 
  19. Blom R, Schrama MJJ, Spitzen J, Weller BFM, van der Linden A, Sikkema RS, et al. Arbovirus persistence in North-Western Europe: Are mosquitoes the only overwintering pathway? One Health. 2022;16:100467.  https://doi.org/10.1016/j.onehlt.2022.100467  PMID: 36531660 
  20. Streng K, Hakze-van der Honing RW, Graham H, van Oort S, de Best PA, Abourashed A, et al. Orthoflavivirus surveillance in the Netherlands: Insights from a serosurvey in horses & dogs and a questionnaire among horse owners. Zoonoses Public Health. 2024;71(8):900-10.  https://doi.org/10.1111/zph.13171  PMID: 39057842 
  21. Streng K, Atama N, Chandler F, Blom R, van der Jeugd H, Schrama M, et al. Sentinel chicken surveillance reveals previously undetected circulation of West Nile virus in the Netherlands. Emerg Microbes Infect. 2024;13(1):2406278.  https://doi.org/10.1080/22221751.2024.2406278  PMID: 39295515 
  22. Münger E, Atama NC, van Irsel J, Blom R, Krol L, van Mastrigt T, et al. One Health approach uncovers emergence and dynamics of Usutu and West Nile viruses in the Netherlands. Nat Commun. 2025;16(1):7883.  https://doi.org/10.1038/s41467-025-63122-w  PMID: 40849294 
  23. Koenraadt CJM, Münger E, Schrama MJJ, Spitzen J, Altundag S, Sikkema RS, et al. Overwintering of Usutu virus in mosquitoes, The Netherlands. Parasit Vectors. 2024;17(1):537.  https://doi.org/10.1186/s13071-024-06620-y  PMID: 39716210 
  24. Streng K. Chapter 4. In: From research to preparedness: A study of zoonotic arboviruses in animals, the Netherlands. PhD thesis. Wageningen: Wageningen University; 2024.  https://doi.org/10.18174/676149 
  25. Braks MAH, Stroo CJ. Westnijlvirus in Nederland: Aanpak Integraal Vectormanagement 2021-2023. [West Nile Virus in the Netherlands: Approach Integrated Vector Management 2021-2023]. Bilthoven: National Institute for Public Health and the Environment; 2021.
  26. van Ewijk C, Feenstra S, ter Bogt-Kappert C, Braks M, Franz E, Geurts van Kessel C, et al. Westnijlvirus in Nederland Surveillance en Respons 2021-2023 Eindrapport. [West Nile Virus in the Netherlands Surveillance and Response 2021-2023 Final Report]. Bilthoven: National Institute for Public Health and the Environment; 2024.
  27. EUR-Lex. Commission Directive 2014/110/EU of 17 December 2014 amending Directive 2004/33/EC as regards temporary deferral criteria for donors of allogeneic blood donations Text with EEA relevance. Official Journal of the European Union. 2014; L366/81. Available from: https://eur-lex.europa.eu/legal-content/EN/TXT/?uri=CELEX%3A32014L0110&qid=1649074114926
  28. Directorate-General for Health and Food Safety. West Nile virus and blood safety introduction to a preparedness plan in Europe. Brussels: European Commission. 6 June 2012. Available from: https://health.ec.europa.eu/document/download/62df551d-a4fa-4a2b-8af5-225506ae9d86_en
  29. Graham H, Streng K, Henneuse H, Fabius L, Holwerda M, van den Wollenberg L, et al. Westnijlvirus dichterbij dan gedacht. [West Nile virus closer than expected]. Tijdschr Diergeneeskd. 2024;149(5):36-9.
  30. Chiari M, Prosperi A, Faccin F, Avisani D, Cerioli M, Zanoni M, et al. West Nile Virus Surveillance in the Lombardy Region, Northern Italy. Transbound Emerg Dis. 2015;62(4):343-9.  https://doi.org/10.1111/tbed.12375  PMID: 25958924 
  31. Rizzoli A, Rosà R, Rosso F, Buckley A, Gould E. West Nile virus circulation detected in northern Italy in sentinel chickens. Vector Borne Zoonotic Dis. 2007;7(3):411-7.  https://doi.org/10.1089/vbz.2006.0626  PMID: 17767411 
  32. Chaintoutis SC, Gewehr S, Mourelatos S, Dovas CI. Serological monitoring of backyard chickens in Central Macedonia-Greece can detect low transmission of West Nile virus in the absence of human neuroinvasive disease cases. Acta Trop. 2016;163:26-31.  https://doi.org/10.1016/j.actatropica.2016.07.018  PMID: 27469618 
  33. Koopmans MPG, Barton Behravesh C, Cunningham AA, Adisasmito WB, Almuhairi S, Bilivogui P, et al. , One Health High-Level Expert Panel. The panzootic spread of highly pathogenic avian influenza H5N1 sublineage 2.3.4.4b: a critical appraisal of One Health preparedness and prevention. Lancet Infect Dis. 2024;24(12):e774-81.  https://doi.org/10.1016/S1473-3099(24)00438-9  PMID: 39134084 
  34. Dos S Ribeiro C, van de Burgwal LHM, Regeer BJ. Overcoming challenges for designing and implementing the One Health approach: A systematic review of the literature. One Health. 2019;7:100085.  https://doi.org/10.1016/j.onehlt.2019.100085  PMID: 31016220 
  35. Parkes MW, Bienen L, Breilh J, Hsu LN, McDonald M, Patz JA, et al. All hands on deck: Transdisciplinary approaches to emerging infectious disease. EcoHealth. 2005;2(4):258-72.  https://doi.org/10.1007/s10393-005-8387-y 
  36. Braks M, Giglio G, Tomassone L, Sprong H, Leslie T. Making vector-borne disease surveillance work: New opportunities from the SDG perspectives. Front Vet Sci. 2019;6:232.  https://doi.org/10.3389/fvets.2019.00232  PMID: 31380399 
  37. Archer BN, Abdelmalik P, Cognat S, Grand PE, Mott JA, Pavlin BI, et al. Defining collaborative surveillance to improve decision making for public health emergencies and beyond. Lancet. 2023;401(10391):1831-4.  https://doi.org/10.1016/S0140-6736(23)01009-7  PMID: 37230104 
  38. Hayman DTS, Adisasmito WB, Almuhairi S, Behravesh CB, Bilivogui P, Bukachi SA, et al. , One Health High-Level Expert Panel (OHHLEP). Developing One Health surveillance systems. One Health. 2023;17:100617.  https://doi.org/10.1016/j.onehlt.2023.100617  PMID: 38024258 
A multidisciplinary approach to the detection of and response to West Nile virus in the Netherlands between 2020 and 2023: best practices, challenges and opportunities
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Pauline+A+de+Best&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Best Pauline A</a>, <a href="/search?value1=Marieta+Braks&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Braks Marieta</a>, <a href="/search?value1=One+Health+PACT+consortium&option1=author&noRedirect=true" class="nonDisambigAuthorLink">One Health PACT consortium</a>, <a href="/search?value1=National+West+Nile+Virus+Response+team%2C+working+group&option1=author&noRedirect=true" class="nonDisambigAuthorLink">National West Nile Virus Response team, working group</a>, <a href="/search?value1=Aura+Timen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Timen Aura</a>, <a href="/search?value1=Reina+S+Sikkema&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sikkema Reina S</a>, <a href="/search?value1=Marion+PG+Koopmans&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Koopmans Marion PG</a></span>. A multidisciplinary approach to the detection of and response to West Nile virus in the Netherlands between 2020 and 2023: best practices, challenges and opportunities. <a href="/content/ecdc">Euro Surveill.</a> 2026;31(10):pii=2500276. <a href="https://doi.org/10.2807/1560-7917.ES.2026.31.10.2500276" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2026.31.10.2500276</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 16 Apr 2025;&nbsp;&nbsp; <span class="generated">Accepted</span>: 08 Aug 2025 </span> </p>
/content/10.2807/1560-7917.ES.2026.31.10.2500276
/content/10.2807/1560-7917.ES.2026.31.10.2500276
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