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SDG 3: Antimicrobial resistance

Abstract

BACKGROUND

Dual penicillin- and ciprofloxacin-resistant causing invasive meningococcal disease (IMD) have recently emerged in association with sequence type (ST) 3587, harbouring ROB-1 β-lactamase ( ) and a mutated DNA gyrase (). These strains pose a threat to current antimicrobial treatment and prophylaxis.

AIM

We aimed to characterise the first dual-resistant ST-3587 isolates harbouring and a mutated identified in Spain.

METHODS

Three isolates encoding were identified in 2024. They were characterised by whole genome sequencing to determine capsular genogroups, ST and genetic antimicrobial resistance markers. Dated phylogenetic analysis was performed alongside global ST-3587 strains.

RESULTS

The three -encoding isolates belonged to ST-3587, genogroup Y, harboured a T91I mutation in and showed resistance to penicillin and ciprofloxacin. These isolates were obtained from urethral, oropharyngeal and blood samples, each from a different patient. According to the dated phylogenetic analysis of ST-3587 and the presence of , two clades were defined: clade I and clade II. Within clade II, subclade II.I was identified, comprising isolates which, in addition to , carried the T91I mutation in . This subclade included the three Spanish isolates, which exhibited close genetic relatedness.

CONCLUSION

This study documents the emergence of ST-3587 with dual resistance in Europe, including a documented urogenital infection by this lineage. Continued surveillance of antimicrobial resistance in , including non-invasive cases, is crucial for timely public health responses and effective IMD prevention strategies.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2026-01-29
2026-02-08
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Emergence of Neisseria meningitidis ST-3587 harbouring bla ROB-1 and exhibiting dual resistance to penicillin and ciprofloxacin, Spain, 2024
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Josep+Roca-Grande&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Roca-Grande Josep</a>, <a href="/search?value1=Albert+Moreno-Mingorance&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Moreno-Mingorance Albert</a>, <a href="/search?value1=Alba+Bell%C3%A9s-Bell%C3%A9s&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bellés-Bellés Alba</a>, <a href="/search?value1=Joaqu%C3%ADn+Burgos&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Burgos Joaquín</a>, <a href="/search?value1=Jordi+C%C3%A0mara&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Càmara Jordi</a>, <a href="/search?value1=Yannick+Hoyos-Mallecot&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hoyos-Mallecot Yannick</a>, <a href="/search?value1=Luc%C3%ADa+L%C3%B3pez-Alcaide&option1=author&noRedirect=true" class="nonDisambigAuthorLink">López-Alcaide Lucía</a>, <a href="/search?value1=Joan+L%C3%B3pez-Madue%C3%B1o&option1=author&noRedirect=true" class="nonDisambigAuthorLink">López-Madueño Joan</a>, <a href="/search?value1=Mayli+Lung&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lung Mayli</a>, <a href="/search?value1=Andrea+Mart%C3%ADn-Nalda&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Martín-Nalda Andrea</a>, <a href="/search?value1=Alba+Mir-Cros&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mir-Cros Alba</a>, <a href="/search?value1=Carmen+Mu%C3%B1oz-Almagro&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muñoz-Almagro Carmen</a>, <a href="/search?value1=Amaresh+P%C3%A9rez-Arg%C3%BCello&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pérez-Argüello Amaresh</a>, <a href="/search?value1=Guillem+Puigsech-Boixeda&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Puigsech-Boixeda Guillem</a>, <a href="/search?value1=M+Dolores+Quesada&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Quesada M Dolores</a>, <a href="/search?value1=Carolina+Sarvis%C3%A9&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sarvisé Carolina</a>, <a href="/search?value1=Aleix+Soler-Garc%C3%ADa&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Soler-García Aleix</a>, <a href="/search?value1=Pere+Soler-Palac%C3%ADn&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Soler-Palacín Pere</a>, <a href="/search?value1=Jes%C3%BAs+Trejo-Zah%C3%ADnos&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Trejo-Zahínos Jesús</a>, <a href="/search?value1=Gloria+Trujillo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Trujillo Gloria</a>, <a href="/search?value1=Bel%C3%A9n+Vi%C3%B1ado&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Viñado Belén</a>, <a href="/search?value1=M+Nieves+Larrosa&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larrosa M Nieves</a>, <a href="/search?value1=Juan+Jos%C3%A9+Gonz%C3%A1lez-L%C3%B3pez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">González-López Juan José</a></span>. Emergence of Neisseria meningitidis ST-3587 harbouring bla ROB-1 and exhibiting dual resistance to penicillin and ciprofloxacin, Spain, 2024. <a href="/content/ecdc">Euro Surveill.</a> 2026;31(4):pii=2500398. <a href="https://doi.org/10.2807/1560-7917.ES.2026.31.4.2500398" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2026.31.4.2500398</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 06 Jun 2025;&nbsp;&nbsp; <span class="generated">Accepted</span>: 26 Aug 2025 </span> </p>
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