Evaluation of immunochromatography tests for detection of novel GII.17 norovirus in stool samples

P Khamrin; A Thongprachum; S Takanashi; S Okitsu; N Maneekarn; S Hayakawa; H Ushijima

doi:10.2807/1560-7917.ES2015.20.28.21185

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Evaluation of immunochromatography tests for detection of novel GII.17 norovirus in stool samples
P Khamrin^1,2,3 , A Thongprachum^2,3 , S Takanashi^2,3 , S Okitsu^2,3 , N Maneekarn¹ , S Hayakawa³ , H Ushijima^2,3
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Affiliations: ¹ Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand ² Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan ³ Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan

Correspondence:
H Ushijima
ushijima-hiroshi jcom.home.ne.jp
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Citation style for this article: Khamrin P, Thongprachum A, Takanashi S, Okitsu S, Maneekarn N, Hayakawa S, Ushijima H. Evaluation of immunochromatography tests for detection of novel GII.17 norovirus in stool samples. Euro Surveill. 2015;20(28):pii=21185. https://doi.org/10.2807/1560-7917.ES2015.20.28.21185 Received: 06 Jul 2015

Abstract

A novel GII.17 norovirus has emerged as a major cause of epidemic and endemic acute gastroenteritis in several countries in Asia. We used a small panel of stool samples in which GII.17 virus had been quantified by real-time RT-PCR to evaluate four commercially available norovirus immunochromatography (IC) kits. At least 10⁸ copies/mL of GII.17 virus were required by each IC kit for a positive result, which is 1,000-fold more than that reported for these assays for GII.4 viruses.