1887
Rapid communication Open Access
Like 0

Abstract

We identified a novel plasmid-mediated colistin-resistance gene in porcine and bovine colistin-resistant that did not contain . The gene, termed , a 1,617 bp phosphoethanolamine transferase harboured on an IncX4 plasmid, has 76.7% nucleotide identity to . Prevalence of in porcine colistin-resistant (11/53) in Belgium was higher than that of (7/53). These data call for an immediate introduction of screening in ongoing molecular epidemiological surveillance of colistin-resistant Gram-negative pathogens.

Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2016.21.27.30280
2016-07-07
2017-11-24
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.27.30280
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/21/27/eurosurv-21-30280-2.html?itemId=/content/10.2807/1560-7917.ES.2016.21.27.30280&mimeType=html&fmt=ahah

References

  1. Liu Y-Y, Wang Y, Walsh TR, Yi L-X, Zhang R, Spencer J, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161-8.  https://doi.org/10.1016/S1473-3099(15)00424-7  PMID: 26603172 
  2. European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0, 2016. EUCAST; 2016. Available from: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_6.0_Breakpoint_table.pdf
  3. Malhotra-Kumar S, Xavier BB, Das AJ, Lammens C, Butaye P, Goossens H. Colistin resistance gene mcr-1 harboured on a multidrug resistant plasmid. Lancet Infect Dis. 2016;16(3):283-4.  https://doi.org/10.1016/S1473-3099(16)00012-8  PMID: 26774247 
  4. Xavier BB, Lammens C, Butaye P, Goossens H, Malhotra-Kumar S. Complete sequence of an IncFII plasmid harbouring the colistin resistance gene mcr-1 isolated from Belgian pig farms. J Antimicrob Chemother. 2016;dkw191.  https://doi.org/10.1093/jac/dkw191  PMID: 27261261 
  5. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455-77.  https://doi.org/10.1089/cmb.2012.0021  PMID: 22506599 
  6. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008;9(1):75.  https://doi.org/10.1186/1471-2164-9-75  PMID: 18261238 
  7. Finn RD, Coggill P, Eberhardt RY, Eddy SR, Mistry J, Mitchell AL, et al. The Pfam protein families database: towards a more sustainable future. Nucleic Acids Res. 2016;44(D1):D279-85.  https://doi.org/10.1093/nar/gkv1344  PMID: 26673716 
  8. Quevillon E, Silventoinen V, Pillai S, Harte N, Mulder N, Apweiler R, et al. InterProScan: protein domains identifier. Nucleic Acids Res. 20051;33(Web Server issue):W116-20. PMID:15980438
  9. Xavier BB, Lammens C, Ruhal R, Kumar-Singh S, Butaye P, Goossens H, et al. Rapid Communication, Eurosurveillance, Identification of a novel plasmid-mediated colistin-resistant gene mcr-2 in E. coli. Supplementary material. [Accessed 7 Jul 2016]. Available from: https://www.uantwerpen.be/en/rg/lab-of-medical-microbiology/projects-and-publications/publications/key-publications/rapid-communication-eurosurveillance/
  10. Siguier P, Perochon J, Lestrade L, Mahillon J, Chandler M. ISfinder: the reference centre for bacterial insertion sequences. Nucleic Acids Res. 2006;34(Database issue):D32-6.  https://doi.org/10.1093/nar/gkj014  PMID: 16381877 
  11. Gokulan K, Khare S, Rooney AW, Han J, Lynne AM, Foley SL. Impact of plasmids, including those encodingVirB4/D4 type IV secretion systems, on Salmonella enterica serovar Heidelberg virulence in macrophages and epithelial cells. PLoS One. 2013;8(10):e77866.  https://doi.org/10.1371/journal.pone.0077866  PMID: 24098597 
  12. Berrisford JM, Baradaran R, Sazanov LA. Structure of bacterial respiratory complex I. Biochim Biophys Acta. 2016;1857(7):892-901.  https://doi.org/10.1016/j.bbabio.2016.01.012  PMID: 26807915 
  13. Wanty C, Anandan A, Piek S, Walshe J, Ganguly J, Carlson RW, et al. The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin. J Mol Biol. 2013;425(18):3389-402.  https://doi.org/10.1016/j.jmb.2013.06.029  PMID: 23810904 
  14. Fernandes MR, Moura Q, Sartori L, Silva KC, Cunha MP, Esposito F, et al. Silent dissemination of colistin-resistant Escherichia coli in South America could contribute to the global spread of the mcr-1 gene. Euro Surveill. 2016;21(17) 16;21(17):pii=30214.
  15. Skov RL, Monnet DL. Plasmid-mediated colistin resistance (mcr-1 gene): three months later, the story unfolds. Euro Surveill. 2016;21(9):30155.  https://doi.org/10.2807/1560-7917.ES.2016.21.9.30155  PMID: 26967914 
  16. Prim N, Rivera A, Rodriguez-Navarro J, Espanol M, Turbau M, Coll P, et al. Detection of mcr-1 colistin resistance gene in polyclonal Escherichia coli isolates in Barcelona, Spain, 2012 to 2015. Euro Surveill. 2016;21(13) (13):pii=30183.
  17. Perreten V, Strauss C, Collaud A, Gerber D. Colistin Resistance Gene mcr-1 in Avian-Pathogenic Escherichia coli in South Africa. Antimicrob Agents Chemother. 2016;60(7):4414-5.  https://doi.org/10.1128/AAC.00548-16  PMID: 27161625 
  18. Rapoport M, Faccone D, Pasteran F, Ceriana P, Albornoz E, Petroni A, et al. First Description of mcr-1-Mediated Colistin Resistance in Human Infections Caused by Escherichia coli in Latin America. Antimicrob Agents Chemother. 2016;60(7):4412-3.  https://doi.org/10.1128/AAC.00573-16  PMID: 27090181 
  19. Izdebski R, Baraniak A, Bojarska K, Urbanowicz P, Fiett J, Pomorska-Wesołowska M, et al. Mobile MCR-1-associated resistance to colistin in Poland. J Antimicrob Chemother. 2016 Jun 20. pii: dkw261.  https://doi.org/10.1093/jac/dkw261  PMID:27330064
  20. McGann P, Snesrud E, Maybank R, Corey B, Ong AC, Clifford R, et al. Escherichia coli Harboring mcr-1 and blaCTX-M on a Novel IncF Plasmid: First Report of mcr-1 in the United States. Antimicrob Agents Chemother. 2016;60(7):4420-1.  https://doi.org/10.1128/AAC.01103-16  PMID: 27230792 
  21. Arcilla MS, van Hattem JM, Matamoros S, Melles DC, Penders J, de Jong MD, et al. Dissemination of the mcr-1 colistin resistance gene. Lancet Infect Dis. 2016;16(2):147-9.  https://doi.org/10.1016/S1473-3099(15)00541-1  PMID: 26711361 
  22. Bernasconi OJ, Kuenzli E, Pires J, Tinguely R, Carattoli A, Hatz C, et al. Travelers Can Import Colistin-Resistant Enterobacteriaceae Including Those Possessing the Plasmid-Mediated mcr-1 Gene. Antimicrob Agents Chemother. 2016 Jun 13. pii: AAC.00731-16. PMID: 27297483
  23. Storm DR, Rosenthal KS, Swanson PE. Polymyxin and related peptide antibiotics. Annu Rev Biochem. 1977;46(1):723-63.  https://doi.org/10.1146/annurev.bi.46.070177.003451  PMID: 197881 
  24. Reynolds CM, Kalb SR, Cotter RJ, Raetz CRH. A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. Identification of the eptB gene and Ca2+ hypersensitivity of an eptB deletion mutant. J Biol Chem. 2005;280(22):21202-11.  https://doi.org/10.1074/jbc.M500964200  PMID: 15795227 
  25. Lo WU, Chow KH, Law PY, Ng KY, Cheung YY, Lai EL, et al. Highly conjugative IncX4 plasmids carrying blaCTX-M in Escherichia coli from humans and food animals. J Med Microbiol. 2014;63(Pt 6):835-40.  https://doi.org/10.1099/jmm.0.074021-0  PMID: 24595536 
  26. Hasman H, Hammerum AM, Hansen F, Hendriksen RS, Olesen B, Agersø Y, et al. Detection of mcr-1 encoding plasmid-mediated colistin-resistant Escherichia coli isolates from human bloodstream infection and imported chicken meat, Denmark 2015. Euro Surveill. 2015;20(49):30085.  https://doi.org/10.2807/1560-7917.ES.2015.20.49.30085  PMID: 26676364 
  27. Vila-Farrés X, Ferrer-Navarro M, Callarisa AE, Martí S, Espinal P, Gupta S, et al. Loss of LPS is involved in the virulence and resistance to colistin of colistin-resistant Acinetobacter nosocomialis mutants selected in vitro. J Antimicrob Chemother. 2015;70(11):2981-6.  https://doi.org/10.1093/jac/dkv244  PMID: 26311838 
  28. Conant G, Wolfe K. GenomeVx. Dublin: University College Dublin. [Accessed 22 Jun 2016]. Available from: http://wolfe.ucd.ie/GenomeVx/
  29. Källberg M, Margaryan G, Wang S, Ma J, Xu J. RaptorX server: a resource for template-based protein structure modeling. Methods Mol Biol. 2014;1137:17-27.  https://doi.org/10.1007/978-1-4939-0366-5_2  PMID: 24573471 
/content/10.2807/1560-7917.ES.2016.21.27.30280
Loading

Data & Media loading...

Comment has been disabled for this content
Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error