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Abstract

In 2012, the European Centre for Disease Prevention and Control (ECDC) initiated external quality assessment (EQA) schemes for molecular typing including the National Public Health Reference Laboratories in Europe. The overall aim for these EQA schemes was to enhance the European surveillance of food-borne pathogens by evaluating and improving the quality and comparability of molecular typing. The EQAs were organised by Statens Serum Institut (SSI) and included subsp. enterica, verocytotoxin-producing (VTEC) and . Inter-laboratory comparable pulsed-field gel electrophoresis (PFGE) images were obtained from 10 of 17 of the participating laboratories for , 15 of 25 for , but only nine of 20 for VTEC. Most problems were related to PFGE running conditions and/or incorrect use of image acquisition. Analysis of the gels was done in good accordance with the provided guidelines. Furthermore, we assessed the multilocus variable-number tandem repeat analysis (MLVA) scheme for Typhimurium. Of 15 laboratories, nine submitted correct results for all analysed strains, and four had difficulties with one strain only. In conclusion, both PFGE and MLVA are prone to variation in quality, and there is therefore a continuous need for standardisation and validation of laboratory performance for molecular typing methods of food-borne pathogens in the human public health sector.

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/content/10.2807/1560-7917.ES.2016.21.50.30429
2016-12-15
2017-12-12
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.50.30429
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References

  1. European Food Safety Authority (EFSA) and European Centre for Disease Prevention and Control (ECDC), 2015. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2013. EFSA Journal. 2015;13(1):3991.
  2. van Walle I. ECDC starts pilot phase for collection of molecular typing data. Euro Surveill. 2013;18(3):20357. PMID: 23351656 
  3. Amato-Gauci A, Ammon A. The surveillance of communicable diseases in the European Union--a long-term strategy (2008-2013). Euro Surveill. 2008;13(26):26. PMID: 18761915 
  4. European Centre for Disease Prevention and Control (ECDC). Surveillance of communicable diseases in Europe – a concept to integrate molecular typing data into EU-level surveillance. Stockholm: ECDC; 2013. Available from: http://www.ecdc.europa.eu/en/publications/publications/surveillance-concept-molecular%20typing-sept2011.pdf
  5. European Centre for Disease Prevention and Control (ECDC). Surveillance of six priority food- and waterborne diseases in the EU/EEA 2006-2009. Stockholm: ECDC; 2013. Available from: http://ecdc.europa.eu/en/publications/Publications/food-and-waterborne-diseases-surveillance-report.pdf
  6. Swaminathan B, Barrett TJ, Hunter SB, Tauxe RVCDC PulseNet Task Force. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis. 2001;7(3):382-9.  https://doi.org/10.3201/eid0703.017303  PMID: 11384513 
  7. Félix B, Niskanen T, Vingadassalon N, Dao TT, Asséré A, Lombard B, et al. Pulsed-field gel electrophoresis proficiency testing trials: toward European harmonization of the typing of food and clinical strains of Listeria monocytogenes. Foodborne Pathog Dis. 2013;10(10):873-81.  https://doi.org/10.1089/fpd.2013.1494  PMID: 24066940 
  8. Li W, Lu S, Cui Z, Cui J, Zhou H, Wang Y, et al. PulseNet China, a model for future laboratory-based bacterial infectious disease surveillance in China. Front Med. 2012;6(4):366-75.  https://doi.org/10.1007/s11684-012-0214-6  PMID: 23124882 
  9. Puohiniemi R, Heiskanen T, Siitonen A. Molecular epidemiology of two international sprout-borne Salmonella outbreaks. J Clin Microbiol. 1997;35(10):2487-91. PMID: 9316894 
  10. Keene WE, Hedberg K, Herriott DE, Hancock DD, McKay RW, Barrett TJ, et al. A prolonged outbreak of Escherichia coli O157:H7 infections caused by commercially distributed raw milk. J Infect Dis. 1997;176(3):815-8.  https://doi.org/10.1086/517310  PMID: 9291342 
  11. Farber JM, Daley EM, MacKie MT, Limerick B. A small outbreak of listeriosis potentially linked to the consumption of imitation crab meat. Lett Appl Microbiol. 2000;31(2):100-4.  https://doi.org/10.1046/j.1365-2672.2000.00775.x  PMID: 10972708 
  12. Graves LM, Swaminathan B. PulseNet standardized protocol for subtyping Listeria monocytogenes by macrorestriction and pulsed-field gel electrophoresis. Int J Food Microbiol. 2001;65(1-2):55-62.  https://doi.org/10.1016/S0168-1605(00)00501-8  PMID: 11322701 
  13. Ribot EM, Fair MA, Gautom R, Cameron DN, Hunter SB, Swaminathan B, et al. Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet. Foodborne Pathog Dis. 2006;3(1):59-67.  https://doi.org/10.1089/fpd.2006.3.59  PMID: 16602980 
  14. Lindstedt BA, Vardund T, Aas L, Kapperud G. Multiple-locus variable-number tandem-repeats analysis of Salmonella enterica subsp. enterica serovar Typhimurium using PCR multiplexing and multicolor capillary electrophoresis. J Microbiol Methods. 2004;59(2):163-72.  https://doi.org/10.1016/j.mimet.2004.06.014  PMID: 15369852 
  15. Lindstedt BA, Heir E, Gjernes E, Kapperud G. DNA fingerprinting of Salmonella enterica subsp. enterica serovar typhimurium with emphasis on phage type DT104 based on variable number of tandem repeat loci. J Clin Microbiol. 2003;41(4):1469-79.  https://doi.org/10.1128/JCM.41.4.1469-1479.2003  PMID: 12682132 
  16. Larsson JT, Torpdahl M, Petersen RF, Sorensen G, Lindstedt BA, Nielsen EM. Development of a new nomenclature for Salmonella typhimurium multilocus variable number of tandem repeats analysis (MLVA). Euro Surveill. 2009;14(15):19174. PMID: 19371515 
  17. Sintchenko V, Wang Q, Howard P, Ha CW, Kardamanidis K, Musto J, et al. Improving resolution of public health surveillance for human Salmonella enterica serovar Typhimurium infection: 3 years of prospective multiple-locus variable-number tandem-repeat analysis (MLVA). BMC Infect Dis. 2012;12(1):78.  https://doi.org/10.1186/1471-2334-12-78  PMID: 22462487 
  18. Torpdahl M, Sørensen G, Lindstedt BA, Nielsen EM. Tandem repeat analysis for surveillance of human Salmonella Typhimurium infections. Emerg Infect Dis. 2007;13(3):388-95.  https://doi.org/10.3201/eid1303.060460  PMID: 17552091 
  19. Paranthaman K, Haroon S, Latif S, Vinnyey N, de Souza V, Welfare W, et al. Emergence of a multidrug-resistant (ASSuTTm) strain of Salmonella enterica serovar Typhimurium DT120 in England in 2011 and the use of multiple-locus variable-number tandem-repeat analysis in supporting outbreak investigations. Foodborne Pathog Dis. 2013;10(10):850-5.  https://doi.org/10.1089/fpd.2013.1513  PMID: 23869962 
  20. Nygård K, Lindstedt BA, Wahl W, Jensvoll L, Kjelsø C, Mølbak K, et al. Outbreak of Salmonella Typhimurium infection traced to imported cured sausage using MLVA-subtyping. Euro Surveill. 2007;12(3):E070315.5. PMID: 17439789 
  21. Nadon CA, Trees E, Ng LK, Møller Nielsen E, Reimer A, Maxwell N, et al. MLVA Harmonization Working Group. Development and application of MLVA methods as a tool for inter-laboratory surveillance. Euro Surveill. 2013;18(35):20565.  https://doi.org/10.2807/1560-7917.ES2013.18.35.20565  PMID: 24008231 
  22. Larsson JT, Torpdahl M, Møller Nielsen EMLVA working group. Proof-of-concept study for successful inter-laboratory comparison of MLVA results. Euro Surveill. 2013;18(35):20566.  https://doi.org/10.2807/1560-7917.ES2013.18.35.20566  PMID: 24008232 
  23. European Centre for Disease Prevention and Control (ECDC). Fourth external quality assessment scheme for Salmonella typing. Stockholm: ECDC; 2013. Available from: http://www.ecdc.europa.eu/en/publications/Publications/salmonella-external-quality-assessment-EQA-scheme-for-typing-2013.pdf
  24. European Centre for Disease Prevention and Control (ECDC). External quality assessment scheme for Listeria typing. Stockholm: ECDC; 2014. Available from: http://www.ecdc.europa.eu/en/publications/Publications/EQA-listeria-monocytogenes.pdf
  25. European Centre for Disease Prevention and Control (ECDC). Fourth external quality assessment scheme for typing of Vero cytotoxin producing Escherichia coli (VTEC). Stockholm: ECDC; 2014. Available from: http://www.ecdc.europa.eu/en/publications/Publications/4th-External-Quality-Assessment-typing-of-verocytotoxin-producing-E.-coli-VTEC-web.pdf
  26. International Standard ISO/IEC 17043:2010 “Conformity assessment – General requirements for proficiency testing”. First edition 01/02/2010.
  27. Centers for Disease Control and Prevention (CDC). One-Day (24-28 h) Standardized Laboratory Protocol for Molecular Subtyping of Escherichia coli O157:H7, non-typhoidal Salmonella serotypes, and Shigella sonnei by Pulsed Field Gel Electrophoresis (PFGE). Atlanta: CDC. [Accessed 3 Mar 2014]. Available from: http://www.cdc.gov/pulsenet/protocols/ecoli_salmonella_shigella_protocols.pdf
  28. PulseNet. One-Day (24-28 h) Standardized Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Pulsed Field Gel Electrophoresis (PFGE). [Accessed 3 Mar 2014]. Available from: http://www.pulsenetinternational.org/assets/PulseNet/uploads/pfge/5.3_2009_PNetStandProtLMonocytogenes.pdf
  29. European Centre for Disease Prevention and Control (ECDC). Laboratory standard operating procedure for MLVA of Salmonella enterica serotype Typhimurium. Stockholm: ECDC; 2011. [Accessed 3 Mar 2014]. Available from: http://ecdc.europa.eu/en/publications/Publications/1109_SOP_Salmonella_Typhimurium_MLVA.pdf
  30. European Centre for Disease Prevention and Control (ECDC). Expert Opinion on the introduction of next-generation typing methods for food- and waterborne diseases in the EU and EEA. Stockholm: ECDC; 2015. Available from: http://ecdc.europa.eu/en/publications/Publications/food-and-waterborne-diseases-next-generation-typing-methods.pdf
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