1887
Surveillance and outbreak report Open Access
Like 0

Abstract

OXA-48-like beta-lactamase producing bacteria are now endemic in several European and Mediterranean countries. Among this carbapenemase family, the OXA-48 and OXA-181 variants predominate, whereas other variants such as OXA-204 are rarely reported. Here, we report the molecular epidemiology of a collection of OXA-204-positive enterobacterial isolates (n = 29) recovered in France between October 2012 and May 2014. This study describes the first outbreak of OXA-204-producing in Europe, involving 12 isolates of an ST90 clone and nine isolates of an ST147 clone. All isolates co-produced the cephalosporinase CMY-4, and 60% of them co-produced the extended-spectrum beta-lactamase CTX-M-15. The gene was located on a 150-kb IncA/C plasmid, isolated from various enterobacterial species in the same patient, indicating a high conjugative ability of this genetic vehicle.

Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2017.22.49.17-00048
2017-12-07
2017-12-11
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.49.17-00048
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/22/49/eurosurv-22-49-3.html?itemId=/content/10.2807/1560-7917.ES.2017.22.49.17-00048&mimeType=html&fmt=ahah

References

  1. Poirel L, Potron A, Nordmann P. OXA-48-like carbapenemases: the phantom menace. J Antimicrob Chemother. 2012;67(7):1597-606.  https://doi.org/10.1093/jac/dks121  PMID: 22499996 
  2. Dortet L, Cuzon G, Nordmann P. Dissemination of carbapenemase-producing Enterobacteriaceae in France, 2012. J Antimicrob Chemother. 2014;69(3):623-7.  https://doi.org/10.1093/jac/dkt433  PMID: 24275115 
  3. Albiger B, Glasner C, Struelens MJ, Grundmann H, Monnet DLEuropean Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) working group. Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015. Euro Surveill. 2015;20(45):30062.  https://doi.org/10.2807/1560-7917.ES.2015.20.45.30062  PMID: 26675038 
  4. Vaux S, Carbonne A, Thiolet JM, Jarlier V, Coignard BRAISIN and Expert Laboratories Groups. Emergence of carbapenemase-producing Enterobacteriaceae in France, 2004 to 2011. Euro Surveill. 2011;16(22):19880.  https://doi.org/10.2807/ese.16.22.19880-en  PMID: 21663708 
  5. Oueslati S, Nordmann P, Poirel L. Heterogeneous hydrolytic features for OXA-48-like β-lactamases. J Antimicrob Chemother. 2015;70(4):1059-63.  https://doi.org/10.1093/jac/dku524  PMID: 25583748 
  6. Potron A, Nordmann P, Lafeuille E, Al Maskari Z, Al Rashdi F, Poirel L. Characterization of OXA-181, a carbapenem-hydrolyzing class D β-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2011;55(10):4896-9.  https://doi.org/10.1128/AAC.00481-11  PMID: 21768505 
  7. Potron A, Nordmann P, Poirel L. Characterization of OXA-204, a carbapenem-hydrolyzing class D β-lactamase from Klebsiella pneumoniae. Antimicrob Agents Chemother. 2013;57(1):633-6.  https://doi.org/10.1128/AAC.01034-12  PMID: 23114766 
  8. Oteo J, Hernández JM, Espasa M, Fleites A, Sáez D, Bautista V, et al. Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain. J Antimicrob Chemother. 2013;68(2):317-21.  https://doi.org/10.1093/jac/dks383  PMID: 23034714 
  9. Potron A, Rondinaud E, Poirel L, Belmonte O, Boyer S, Camiade S, et al. Genetic and biochemical characterisation of OXA-232, a carbapenem-hydrolysing class D β-lactamase from Enterobacteriaceae. Int J Antimicrob Agents. 2013;41(4):325-9.  https://doi.org/10.1016/j.ijantimicag.2012.11.007  PMID: 23305656 
  10. Potron A, Poirel L, Dortet L, Nordmann P. Characterisation of OXA-244, a chromosomally-encoded OXA-48-like β-lactamase from Escherichia coli. Int J Antimicrob Agents. 2016;47(1):102-3.  https://doi.org/10.1016/j.ijantimicag.2015.10.015  PMID: 26655033 
  11. Poirel L, Castanheira M, Carrër A, Rodriguez CP, Jones RN, Smayevsky J, et al. OXA-163, an OXA-48-related class D β-lactamase with extended activity toward expanded-spectrum cephalosporins. Antimicrob Agents Chemother. 2011;55(6):2546-51.  https://doi.org/10.1128/AAC.00022-11  PMID: 21422200 
  12. Gomez S, Pasteran F, Faccone D, Bettiol M, Veliz O, De Belder D, et al. Intrapatient emergence of OXA-247: a novel carbapenemase found in a patient previously infected with OXA-163-producing Klebsiella pneumoniae. Clin Microbiol Infect. 2013;19(5):E233-5.  https://doi.org/10.1111/1469-0691.12142  PMID: 23402333 
  13. Dortet L, Oueslati S, Jeannot K, Tandé D, Naas T, Nordmann P. Genetic and biochemical characterization of OXA-405, an OXA-48-type extended-spectrum β-lactamase without significant carbapenemase activity. Antimicrob Agents Chemother. 2015;59(7):3823-8.  https://doi.org/10.1128/AAC.05058-14  PMID: 25870062 
  14. Charfi K, Mansour W, Khalifa AB, Mastouri M, Aouni M, Mammeri H. Emergence of OXA-204 β-lactamase in Tunisia. Diagn Microbiol Infect Dis. 2015;82(4):314-7.  https://doi.org/10.1016/j.diagmicrobio.2015.04.003  PMID: 26001616 
  15. Grami R, Mansour W, Ben Haj Khalifa A, Dahmen S, Chatre P, Haenni M, et al. Emergence of ST147 Klebsiella pneumoniae producing OXA-204 carbapenemase in a University Hospital, Tunisia. Microb Drug Resist. 2016;22(2):137-40.  https://doi.org/10.1089/mdr.2014.0278  PMID: 26447939 
  16. European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 6.0. Växjö: EUCAST; 2016. Available from: http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_6.0_Breakpoint_table.pdf
  17. Poirel L, Dortet L, Bernabeu S, Nordmann P. Genetic features of blaNDM-1-positive Enterobacteriaceae. Antimicrob Agents Chemother. 2011;55(11):5403-7.  https://doi.org/10.1128/AAC.00585-11  PMID: 21859933 
  18. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis. 2011;70(1):119-23.  https://doi.org/10.1016/j.diagmicrobio.2010.12.002  PMID: 21398074 
  19. Diancourt L, Passet V, Verhoef J, Grimont PA, Brisse S. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. J Clin Microbiol. 2005;43(8):4178-82.  https://doi.org/10.1128/JCM.43.8.4178-4182.2005  PMID: 16081970 
  20. Klebsiella sequence typing. Paris: Institut Pasteur. [Accessed: 15 Jan 2017). Available from: http://bigsdb.pasteur.fr/klebsiella/klebsiella.html
  21. EnteroBase. Escherichia coli MLST database. Warwick: EnteroBase. [ Accessed: 15 Jan 2017). Available from: http://mlst.ucc.ie/mlst/dbs/Ecoli
  22. Kieser T. Factors affecting the isolation of CCC DNA from Streptomyces lividans and Escherichia coli. Plasmid. 1984;12(1):19-36.  https://doi.org/10.1016/0147-619X(84)90063-5  PMID: 6387733 
  23. Potron A, Poirel L, Nordmann P. Derepressed transfer properties leading to the efficient spread of the plasmid encoding carbapenemase OXA-48. Antimicrob Agents Chemother. 2014;58(1):467-71.  https://doi.org/10.1128/AAC.01344-13  PMID: 24189247 
  24. Carattoli A, Bertini A, Villa L, Falbo V, Hopkins KL, Threlfall EJ. Identification of plasmids by PCR-based replicon typing. J Microbiol Methods. 2005;63(3):219-28.  https://doi.org/10.1016/j.mimet.2005.03.018  PMID: 15935499 
  25. Circulaire n°2003-591 du 17/12/2003 relative aux modalités de traitement manuel pour la désinfection des endoscopes non autoclavables dans les lieux de soins. [Circular no. 2003-591 of 17 Dec 2003 relating to the manual treatment modalities for the disinfection of non-autoclavable endoscopes in places of care]. Paris: Ministere de la Sante, de la Famille et des Personnes handicapees; 2003. French. Available from: http://circulaires.legifrance.gouv.fr/pdf/2009/04/cir_8584.pdf
  26. Eléments d'assurance qualité en hygiène relatifs au contrôle microbiologique des endoscopes et à la traçabilité en endoscopie. [Elements of quality assurance in hygiene relating to microbiological control of endoscopes and traceability in endoscopy]. Paris: Ministère des Affaires sociales et de la Santé; 2007. Franch. Available from: http://social-sante.gouv.fr/IMG/pdf/microbio_endoscopes-2.pdf
  27. Potron A, Poirel L, Rondinaud E, Nordmann P. Intercontinental spread of OXA-48 β-lactamase-producing Enterobacteriaceae over a 11-year period, 2001 to 2011. Euro Surveill. 2013;18(31):20549.  https://doi.org/10.2807/1560-7917.ES2013.18.31.20549  PMID: 23929228 
  28. Mushtaq S, Irfan S, Sarma JB, Doumith M, Pike R, Pitout J, et al. Phylogenetic diversity of Escherichia coli strains producing NDM-type carbapenemases. J Antimicrob Chemother. 2011;66(9):2002-5.  https://doi.org/10.1093/jac/dkr226  PMID: 21669947 
  29. Österblad M, Kirveskari J, Hakanen AJ, Tissari P, Vaara M, Jalava J. Carbapenemase-producing Enterobacteriaceae in Finland: the first years (2008-11). J Antimicrob Chemother. 2012;67(12):2860-4.  https://doi.org/10.1093/jac/dks299  PMID: 22855858 
  30. Gamal D, Fernández-Martínez M, Salem D, El-Defrawy I, Montes LA, Ocampo-Sosa AA, et al. Carbapenem-resistant Klebsiella pneumoniae isolates from Egypt containing blaNDM-1 on IncR plasmids and its association with rmtF. Int J Infect Dis. 2016;43:17-20.  https://doi.org/10.1016/j.ijid.2015.12.003  PMID: 26686939 
  31. Shin J, Baek JY, Cho SY, Huh HJ, Lee NY, Song JH, et al. blaNDM-5-bearing IncFII-type plasmids of Klebsiella pneumoniae sequence type 147 transmitted by cross-border transfer of a patient. Antimicrob Agents Chemother. 2016;60(3):1932-4.  https://doi.org/10.1128/AAC.02722-15  PMID: 26824953 
  32. Rodrigues C, Machado E, Ramos H, Peixe L, Novais Â. Expansion of ESBL-producing Klebsiella pneumoniae in hospitalized patients: a successful story of international clones (ST15, ST147, ST336) and epidemic plasmids (IncR, IncFIIK). Int J Med Microbiol. 2014;304(8):1100-8.  https://doi.org/10.1016/j.ijmm.2014.08.003  PMID: 25190354 
  33. Bathoorn E, Tsioutis C, da Silva Voorham JM, Scoulica EV, Ioannidou E, Zhou K, et al. Emergence of pan-resistance in KPC-2 carbapenemase-producing Klebsiella pneumoniae in Crete, Greece: a close call. J Antimicrob Chemother. 2016;71(5):1207-12.  https://doi.org/10.1093/jac/dkv467  PMID: 26817488 
  34. Aibinu I, Odugbemi T, Koenig W, Ghebremedhin B. Sequence type ST131 and ST10 complex (ST617) predominant among CTX-M-15-producing Escherichia coli isolates from Nigeria. Clin Microbiol Infect. 2012;18(3):E49-51.  https://doi.org/10.1111/j.1469-0691.2011.03730.x  PMID: 22192452 
  35. Torres-González P, Bobadilla-Del Valle M, Tovar-Calderón E, Leal-Vega F, Hernández-Cruz A, Martínez-Gamboa A, et al. Outbreak caused by Enterobacteriaceae harboring NDM-1 metallo-β-lactamase carried in an IncFII plasmid in a tertiary care hospital in Mexico City. Antimicrob Agents Chemother. 2015;59(11):7080-3.  https://doi.org/10.1128/AAC.00055-15  PMID: 26282410 
  36. Seni J, Falgenhauer L, Simeo N, Mirambo MM, Imirzalioglu C, Matee M, et al. Multiple ESBL-producing Escherichia coli sequence types carrying quinolone and aminoglycoside resistance genes circulating in companion and domestic farm animals in Mwanza, Tanzania, harbor commonly occurring plasmids. Front Microbiol. 2016;11;7:142.
  37. Ben Said L, Jouini A, Alonso CA, Klibi N, Dziri R, Boudabous A, et al. Characteristics of extended-spectrum β-lactamase (ESBL)- and pAmpC beta-lactamase-producing Enterobacteriaceae of water samples in Tunisia. Sci Total Environ. 2016;550:1103-9.  https://doi.org/10.1016/j.scitotenv.2016.01.042  PMID: 26871556 
  38. Information de sécurité. Ecouvillonnage de l’érecteur du Duodénoscope TJF Q180V. [Safety information. Swabbing the TJF Q180V duodenoscope erector]. Rungis Cedex: OLYMPUS France S.A.S; 2014. French. Available from: http://ansm.sante.fr/content/download/65965/844017/version/2/file/mes-140807-Duodenoscope-Olympus.pdf
  39. Mise à jour du mode d’emploi et nouvelles de nettoyage pour le duodénoscope OLYMPUS TJF - Q180V. Guide de nettoyage et écouvillons de nettoyage MAJ-1888/MyBrush. [Update of the user manual and cleaning news for the OLYMPUS TJF - Q180V duodenoscope - MAJ-1888. MyBrush cleaning guide and cleaning swabs]. Rungis Cedex: OLYMPUS France S.A.S; 2015. French. Available from: http://ansm.sante.fr/content/download/78191/991251/version/1/file/mes_150619_olympus.pdf
  40. Muscarella LF. Risk of transmission of carbapenem-resistant Enterobacteriaceae and related "superbugs" during gastrointestinal endoscopy. World J Gastrointest Endosc. 2014;6(10):457-74.  https://doi.org/10.4253/wjge.v6.i10.457  PMID: 25324917 
  41. Arana DM, Saez D, García-Hierro P, Bautista V, Fernández-Romero S, Ángel de la Cal M, et al. Concurrent interspecies and clonal dissemination of OXA-48 carbapenemase. Clin Microbiol Infect. 2015;21(2):148.e1-4.  https://doi.org/10.1016/j.cmi.2014.07.008  PMID: 25596781 
  42. Dortet L, Naas T, Boytchev I, Fortineau N. Endoscopy-associated transmission of carbapenemase-producing Enterobacteriaceae: return of 5 years’ experience. Endoscopy. 2015;47(6):561.  https://doi.org/10.1055/s-0034-1392098  PMID: 26030891 
  43. Nakano R, Okamoto R, Nagano N, Inoue M. Resistance to gram-negative organisms due to high-level expression of plasmid-encoded ampC β-lactamase blaCMY-4 promoted by insertion sequence ISEcp1. J Infect Chemother. 2007;13(1):18-23.  https://doi.org/10.1007/s10156-006-0483-6  PMID: 17334724 
  44. Poirel L, Decousser JW, Nordmann P. Insertion sequence ISEcp1B is involved in expression and mobilization of a bla(CTX-M) β-lactamase gene. Antimicrob Agents Chemother. 2003;47(9):2938-45.  https://doi.org/10.1128/AAC.47.9.2938-2945.2003  PMID: 12936998 
/content/10.2807/1560-7917.ES.2017.22.49.17-00048
Loading

Data & Media loading...

Comment has been disabled for this content
Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error