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SDG 3: Tuberculosis

Abstract

Background

The EUSeqMyTB project, conducted in 2020, used whole genome sequencing (WGS) for surveillance of drug-resistant in the European Union/European Economic Area (EU/EEA) and identified 56 internationally clustered multidrug-resistant (MDR) tuberculosis (TB) clones.

Aim

We aimed to define and establish a rapid and computationally simple screening method to identify probable members of the main cross-border MDR-TB clusters in WGS data to facilitate their identification and track their future spread.

Methods

We screened 34 of the larger cross-border clusters identified in the EuSeqMyTB pilot study (2017–19) for characteristic single nucleotide polymorphism (SNP) signatures that could identify and define members of each cluster. We also linked this analysis with published clusters identified in previous studies and identified more distant genetic relationships between some of the current clusters.

Results

A panel of 30 characteristic SNPs is presented that can be used as an initial (routine) screen for members of each cluster. For four of the clusters, no unique defining SNP could be identified; three of these are closely related (within approximately 20 SNPs) to one or more other clusters and likely represent a single established MDR-TB clade composed of multiple recent subclusters derived from the previously described ECDC0002 cluster.

Conclusion

The identified SNP signatures can be integrated into routine pipelines and contribute to the more effective monitoring, rapid and widespread screening for TB. This SNP panel will also support accurate communication between laboratories about previously identified internationally transmitted MDR-TB genotypes.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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/content/10.2807/1560-7917.ES.2024.29.12.2300583
2024-03-21
2024-04-27
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2024.29.12.2300583
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Characteristic SNPs defining the major multidrug-resistant Mycobacterium tuberculosis clusters identified by EuSeqMyTB to support routine surveillance, EU/EEA, 2017 to 2019
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Albert+J+de+Neeling&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Neeling Albert J</a>, <a href="/search?value1=Elisa+Tagliani&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tagliani Elisa</a>, <a href="/search?value1=Csaba+K%C3%B6dm%C3%B6n&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ködmön Csaba</a>, <a href="/search?value1=Marieke+J+van+der+Werf&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van der Werf Marieke J</a>, <a href="/search?value1=Dick+van+Soolingen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">van Soolingen Dick</a>, <a href="/search?value1=Daniela+Maria+Cirillo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cirillo Daniela Maria</a>, <a href="/search?value1=Richard+M+Anthony&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anthony Richard M</a></span>. Characteristic SNPs defining the major multidrug-resistant Mycobacterium tuberculosis clusters identified by EuSeqMyTB to support routine surveillance, EU/EEA, 2017 to 2019. <a href="/content/ecdc">Euro Surveill.</a> 2024;29(12):pii=2300583. <a href="https://doi.org/10.2807/1560-7917.ES.2024.29.12.2300583" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2024.29.12.2300583</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 23 Oct 2023;&nbsp;&nbsp; <span class="generated">Accepted</span>: 01 Mar 2024 </span> </p>
/content/10.2807/1560-7917.ES.2024.29.12.2300583
/content/10.2807/1560-7917.ES.2024.29.12.2300583
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