1887
Articles Open Access
Like 0
This item has no PDF Download

Abstract

It was announced on 11 July that infectious poliovirus cDNA had been synthesised de novo in a laboratory for the first time (1). Scientists synthesised full length poliovirus cDNA by assembling oligonucleotides of plus and minus strand polarity, and then transcribed them into viral RNA with RNA polymerase. The viral RNA was translated and the synthesis of infectious poliovirus replicated. Transgenic mice injected intracerebrally with this virus developed flaccid paralysis or death, resembling the disease caused by wild poliovirus. The results confirm that the paralysis was caused by the synthetic virus, although a larger inoculum of the synthetic virus was needed to cause paralysis than was the case for wild virus.

Loading

Article metrics loading...

/content/10.2807/esw.06.29.01911-en
2002-07-18
2024-10-07
http://instance.metastore.ingenta.com/content/10.2807/esw.06.29.01911-en
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurvweeklyarchive/6/29/art01911-en.htm?itemId=/content/10.2807/esw.06.29.01911-en&mimeType=html&fmt=ahah
Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error