- Dorina Timofte1,2,3,4, Carmen Valentina Panzaru4,5,7, Iuliana Elena Maciuca1,4, Maria Dan7, Anca Delia Mare6, Adrian Man6, Felicia Toma6
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View Affiliations Hide AffiliationsAffiliations: 1 School of Veterinary Science, University of Liverpool, Leahurst Campus, Neston, United Kingdom 2 Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom 3 Faculty of Veterinary Medicine, University of Agronomical Sciences and Veterinary Medicine, Iasi, Romania 4 These authors contributed equally to this work 5 Microbiology Department, University of Medicine and Pharmacy ‘Grigore T. Popa’, Iasi, Romania 6 Microbiology Department, University of Medicine and Pharmacy Targu Mures, Targu Mureș, Romania 7 Institute of Cardiovascular Diseases, Iasi, RomaniaDorina Timofted.timofte liverpool.ac.uk
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Citation style for this article: Timofte Dorina, Panzaru Carmen Valentina, Maciuca Iuliana Elena, Dan Maria, Mare Anca Delia, Man Adrian, Toma Felicia. Active surveillance scheme in three Romanian hospitals reveals a high prevalence and variety of carbapenamase-producing Gram-negative bacteria: a pilot study, December 2014 to May 2015. Euro Surveill. 2016;21(25):pii=30262. https://doi.org/10.2807/1560-7917.ES.2016.21.25.30262 Received: 30 Sep 2015; Accepted: 12 Feb 2016
Active surveillance scheme in three Romanian hospitals reveals a high prevalence and variety of carbapenamase-producing Gram-negative bacteria: a pilot study, December 2014 to May 2015
Abstract
We report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing Gram-negative bacteria (CP-GNB) in Romanian hospitals. During a pilot study from December 2014 to May 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. The study revealed a high prevalence of CP-GNB detected in 22/27 and 28/38 of the carbapenem non-susceptible isolates from Hospitals 1 and 3, respectively. CP-GNB identified through faecal screening included NDM-1-producing Serratia marcescens and Klebsiella pneumoniae, OXA-48-producing K. pneumoniae and OXA-23-producing Acinetobacter baumannii. The distribution of the CP-GNB varied between the hospitals, with NDM-1-producing S. marcescens and K. pneumoniae being prevalent in the north-central part of the country and OXA-23/24-producing A. baumannii, OXA-48-producing K.pneumoniae, Morganella morganii and VIM-2-producing Escherichia coli/Pseudomonas aeruginosa detected in the north-east of the country. Conjugation studies showed that carbapenem resistance was transferable and PCR-based replicon typing identified blaNDM-1 on IncFIIs in S. marcescens and K. pneumoniae from Hospital 1 and blaOXA-48 on IncL plasmids in all Klebsiella spp. isolates from Hospitals 1 and 3. Our findings underline the importance of active surveillance for detection of CP-GNB asymptomatic faecal carriers and suggest a likely endemic spread of CP-GNB in Romania.

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