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Abstract

We report the findings of an active surveillance scheme for detection of asymptomatic carriers with carbapenemase-producing Gram-negative bacteria (CP-GNB) in Romanian hospitals. During a pilot study from December 2014 to May 2015, faecal cultures were screened in three hospitals (two large, one medium-size) for patients newly admitted to selected wards or inpatients transferred from other wards to an intensive-care unit. The study revealed a high prevalence of CP-GNB detected in 22/27 and 28/38 of the carbapenem non-susceptible isolates from Hospitals 1 and 3, respectively. CP-GNB identified through faecal screening included NDM-1-producing and , OXA-48-producing and OXA-23-producing The distribution of the CP-GNB varied between the hospitals, with NDM-1-producing and being prevalent in the north-central part of the country and OXA-23/24-producing , OXA-48-producing and VIM-2-producing / detected in the north-east of the country. Conjugation studies showed that carbapenem resistance was transferable and PCR-based replicon typing identified on IncFIIs in and from Hospital 1 and on IncL plasmids in all spp. isolates from Hospitals 1 and 3. Our findings underline the importance of active surveillance for detection of CP-GNB asymptomatic faecal carriers and suggest a likely endemic spread of CP-GNB in Romania.

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/content/10.2807/1560-7917.ES.2016.21.25.30262
2016-06-23
2020-05-25
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.25.30262
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Active surveillance scheme in three Romanian hospitals reveals a high prevalence and variety of carbapenamase-producing Gram-negative bacteria: a pilot study, December 2014 to May 2015
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Dorina+Timofte&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Timofte Dorina</a>, <a href="/search?value1=Carmen+Valentina+Panzaru&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Panzaru Carmen Valentina</a>, <a href="/search?value1=Iuliana+Elena+Maciuca&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Maciuca Iuliana Elena</a>, <a href="/search?value1=Maria+Dan&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Dan Maria</a>, <a href="/search?value1=Anca+Delia+Mare&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mare Anca Delia</a>, <a href="/search?value1=Adrian+Man&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Man Adrian</a>, <a href="/search?value1=Felicia+Toma&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Toma Felicia</a></span>. Active surveillance scheme in three Romanian hospitals reveals a high prevalence and variety of carbapenamase-producing Gram-negative bacteria: a pilot study, December 2014 to May 2015. <a href="/content/ecdc">Euro Surveill.</a> 2016;21(25):pii=30262. <a href="https://doi.org/10.2807/1560-7917.ES.2016.21.25.30262" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2016.21.25.30262</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 30 Sep 2015;&nbsp;&nbsp; <span class="generated">Accepted</span>: 12 Feb 2016 </span> </p>
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