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Clostridium difficile PCR ribotypes 001 and 176 – the common denominator of C. difficile infection epidemiology in the Czech Republic, 2014
- Marcela Krutova1,2 , Jana Matejkova1 , Ed J Kuijper3 , Pavel Drevinek1 , Otakar Nyc1 , Czech Clostridium difficile study group4
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View Affiliations Hide AffiliationsAffiliations: 1 Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, Czech Republic 2 DNA Laboratory, Department of Paediatric Neurology, 2nd Faculty of Medicine, Charles University in Prague and University Hospital Motol, Czech Republic 3 Department of Medical Microbiology, Leiden University Medical Centre, Leiden, the Netherlands 4 Members of the study group are listed at the end of the articleMarcela Krutovamarcela.krutova seznam.cz
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Citation style for this article: Krutova Marcela, Matejkova Jana, Kuijper Ed J, Drevinek Pavel, Nyc Otakar, Czech Clostridium difficile study group. Clostridium difficile PCR ribotypes 001 and 176 – the common denominator of C. difficile infection epidemiology in the Czech Republic, 2014. Euro Surveill. 2016;21(29):pii=30296. https://doi.org/10.2807/1560-7917.ES.2016.21.29.30296 Received: 27 Jul 2015; Accepted: 27 Nov 2015
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Abstract
In 2014, 18 hospitals in the Czech Republic participated in a survey of the incidence of Clostridium difficile infections (CDI) in the country. The mean CDI incidence was 6.1 (standard deviation (SD):7.2) cases per 10,000 patient bed-days and 37.8 cases (SD: 41.4) per 10,000 admissions. The mean CDI testing frequency was 39.5 tests (SD: 25.4) per 10,000 patient bed-days and 255.8 tests (SD: 164.0) per 10,000 admissions. A total of 774 C. difficile isolates were investigated, of which 225 (29%) belonged to PCR ribotype 176, and 184 isolates (24%) belonged to PCR ribotype 001. Multilocus variable-number tandem repeat analysis (MLVA) revealed 27 clonal complexes formed by 84% (190/225) of PCR ribotype 176 isolates, and 14 clonal complexes formed by 77% (141/184) of PCR ribotype 001 isolates. Clonal clusters of PCR ribotypes 176 and 001 were observed in 11 and 7 hospitals, respectively. Our data demonstrate the spread of two C. difficile PCR ribotypes within 18 hospitals in the Czech Republic, stressing the importance of standardising CDI testing protocols and implementing mandatory CDI surveillance in the country.

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