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Abstract

The present study aimed to determine the role of human parvovirus В19 (B19V) as an aetiological agent in measles and rubella negative fever/rash patients from Bulgaria between 2004 and 2013. A total of 1,266 sera from all over the country were tested for B19V IgM antibodies and all positives were further investigated by polymerase chain reaction (PCR). Overall, 280 sera (22%) were B19V IgM positive and 227 of these (81%) were also PCR positive. The highest number of IgM positives was found among five to nine year-old children (27%). Eight infected women gave birth to healthy children; one fetus was aborted with hydrops fetalis. Of the 55 genetic sequences obtained, 54 belonged to genotype 1a and one grouped as a genotype 2 outlier. Phylogenetic analysis of all available genotype 2 sequences covering the 994 nucleotide non-structural protein 1(NS1)/capsid viral protein 1 (VP1) unique region junction, showed that only one other sequence grouped with the outlier strain, forming a clearly distinct and well-supported cluster of genotype 2 (between-group genetic distance: 3.32%). In accordance with B19V nomenclature, this cluster may represent a new subgenotype 2b. The study showed that B19V infections may be falsely identified as rubella or measles in ca 22% of cases, emphasising the need for laboratory confirmation.

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/content/10.2807/1560-7917.ES.2016.21.4.30116
2016-01-28
2021-01-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.4.30116
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References

  1. Cossart YE, Field AM, Cant B, Widdows D. Parvovirus-like particles in human sera. Lancet. 1975;305(7898):72-3.  https://doi.org/10.1128/JVI.76.18.9124-9134.2002  PMID: 12186896 
  2. Servant A, Laperche S, Lallemand F, Marinho V, De Saint Maur G, Meritet JF, et al. Genetic diversity within human erythroviruses: identification of three genotypes. J Virol. 2002;76(18):9124-34.  https://doi.org/10.1128/JVI.76.18.9124-9134.2002  PMID: 12186896 
  3. Nicolay N, Cotter S. Clinical and epidemiological aspects of parvovirus B19 infections in Ireland, January 1996-June 2008. Euro Surveill. 2009;14(25):19249. PMID: 19555594 
  4. Young NS, Brown KE. Parvovirus B19. N Engl J Med. 2004;350(6):586-97.  https://doi.org/10.1056/NEJMra030840  PMID: 14762186 
  5. Toshev A, Ivanova S, Kovaleva V, Andonova L, Mihneva Z. Detection of Human parvovirus B19 (HPVB19) in serum samples from fever-rash ill individuals during the rubella outbreak (2005) in Bulgaria. Biotechnol Biotechnol Equip.2014;28(6):1103-7.  https://doi.org/10.1080/13102818.2014.967746  PMID: 26019596 
  6. Gonçalves G, Correia AM, Palminha P, Rebelo-Andrade H, Alves A. Outbreaks caused by parvovirus B19 in three Portuguese schools. Euro Surveill. 2005;10(6):121-4. PMID: 16077212 
  7. World Health Organization (WHO). Strategic plan for measles and congenital rubella infection in the European Region of WHO 2003. Geneva: WHO. Available from: http://www.euro.who.int/__data/assets/pdf_file/0020/79022/E81567.pdf
  8. World Health Organization (WHO). Surveillance guidelines for measles, rubella and congenital rubella syndrome in the WHO Europe region. Copenhagen, update December 2012. Geneva: WHO. Available from: http://www.euro.who.int/__data/assets/pdf_file/0018/79020/e93035-2013.pdf?ua=1
  9. Siennicka J, Trzcińska A. Comparison of three enzyme immunoassays used to detect human parvovirus B19-specific IgM antibodies in sera of people suspected of measles. Med Sci Monit. 2010;16(5):BR154-9. PMID: 20424545 
  10. Molenaar-de Backer MW, Lukashov VV, van Binnendijk RS, Boot HJ, Zaaijer HL. Global co-existence of two evolutionary lineages of parvovirus B19 1a, different in genome-wide synonymous positions. PLoS ONE. 2012;7(8):e43206.  https://doi.org/10.1371/journal.pone.0043206  PMID: 22912828 
  11. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24(8):1596-9.  https://doi.org/10.1093/molbev/msm092  PMID: 17488738 
  12. Marinova L, Kojouharova M, Mihneva Z. An ongoing measles outbreak in Bulgaria, 2009. Euro Surveill. 2009;14(26):19259. PMID: 19573512 
  13. Marinova L, Muscat M, Mihneva Z, Kojouharova M. An update on an ongoing measles outbreak in Bulgaria, April-November 2009. Euro Surveill. 2009;14(50):19442. PMID: 20070938 
  14. Hübschen JM, Mihneva Z, Mentis AF, Schneider F, Aboudy Y, Grossman Z, et al. Phylogenetic analysis of human parvovirus b19 sequences from eleven different countries confirms the predominance of genotype 1 and suggests the spread of genotype 3b. J Clin Microbiol. 2009;47(11):3735-8.  https://doi.org/10.1128/JCM.01201-09  PMID: 19741071 
  15. Ramsay M, Reacher M, O’Flynn C, Buttery R, Hadden F, Cohen B, et al. Causes of morbilliform rash in a highly immunised English population. Arch Dis Child. 2002;87(3):202-6.  https://doi.org/10.1136/adc.87.3.202  PMID: 12193426 
  16. Yermalovich MA, Hübschen JM, Semeiko GV, Samoilovich EO, Muller CP. Human parvovirus B19 surveillance in patients with rash and fever from Belarus. J Med Virol. 2012;84(6):973-8.  https://doi.org/10.1002/jmv.23294  PMID: 22499021 
  17. Davidkin I, Valle M, Peltola H, Hovi T, Paunio M, Roivainen M, et al. Etiology of measles- and rubella-like illnesses in measles, mumps, and rubella-vaccinated children. J Infect Dis. 1998;178(6):1567-70.  https://doi.org/10.1086/314513  PMID: 9815205 
  18. Mossong J, Hens N, Friederichs V, Davidkin I, Broman M, Litwinska B, et al. Parvovirus B19 infection in five European countries: seroepidemiology, force of infection and maternal risk of infection. Epidemiol Infect. 2008;136(8):1059-68.  https://doi.org/10.1017/S0950268807009661  PMID: 17956642 
  19. Yermalovich MA, Semeiko GV, Samoilovich EO, Svirchevskaya EY, Muller CP, Hübschen JM. Etiology of maculopapular rash in measles and rubella suspected patients from Belarus. PLoS ONE. 2014;9(10):e111541.  https://doi.org/10.1371/journal.pone.0111541  PMID: 25356680 
  20. Handysides S. Parvovirus B19 and intrauterine fetal death – not always associated with hydrops. Euro Surveill. 2001;5(20):1751.
  21. Miller E, Fairley CK, Cohen BJ, Seng C. Immediate and long term outcome of human parvovirus B19 infection in pregnancy. Br J Obstet Gynaecol. 1998;105(2):174-8.  https://doi.org/10.1111/j.1471-0528.1998.tb10048.x  PMID: 9501782 
  22. Wermelinger MC, Oelemann WM, Lima de Mendonça MC, Naveca FG, von Hubinger MG. Detection of human parvovirus B19 infection: a study of 212 suspected cases in the state of Rio de Janeiro, Brazil. J Clin Virol. 2002;25(2):223-30.  https://doi.org/10.1016/S1386-6532(02)00014-8  PMID: 12367658 
  23. Hoebe CJ, Claas EC, Steenbergen JE, Kroes AC. Confirmation of an outbreak of parvovirus B19 in a primary school using IgM ELISA and PCR on thumb prick blood samples. J Clin Virol. 2002;25(3):303-7.  https://doi.org/10.1016/S1386-6532(02)00021-5  PMID: 12423694 
  24. Corcoran C, Hardie D, Yeats J, Smuts H. Genetic variants of human parvovirus B19 in South Africa: cocirculation of three genotypes and identification of a novel subtype of genotype 1. J Clin Microbiol. 2010;48(1):137-42.  https://doi.org/10.1128/JCM.00610-09  PMID: 19923483 
  25. Heegaard ED, Panum Jensen I, Christensen J. Novel PCR assay for differential detection and screening of erythrovirus B19 and erythrovirus V9. J Med Virol. 2001;65(2):362-7.  https://doi.org/10.1002/jmv.2042  PMID: 11536245 
  26. Hokynar K, Norja P, Laitinen H, Palomäki P, Garbarg-Chenon A, Ranki A, et al. Detection and differentiation of human parvovirus variants by commercial quantitative real-time PCR tests. J Clin Microbiol. 2004;42(5):2013-9.  https://doi.org/10.1128/JCM.42.5.2013-2019.2004  PMID: 15131163 
  27. Cohen BJ, Gandhi J, Clewley JP. Genetic variants of parvovirus B19 identified in the United Kingdom: implications for diagnostic testing. J Clin Virol. 2006;36(2):152-5.  https://doi.org/10.1016/j.jcv.2006.01.011  PMID: 16569510 
  28. Liefeldt L, Plentz A, Klempa B, Kershaw O, Endres AS, Raab U, et al. Recurrent high level parvovirus B19/genotype 2 viremia in a renal transplant recipient analyzed by real-time PCR for simultaneous detection of genotypes 1 to 3. J Med Virol. 2005;75(1):161-9.  https://doi.org/10.1002/jmv.20251  PMID: 15543575 
  29. Blümel J, Eis-Hübinger AM, Stühler A, Bönsch C, Gessner M, Löwer J. Characterization of Parvovirus B19 genotype 2 in KU812Ep6 cells. J Virol. 2005;79(22):14197-206.  https://doi.org/10.1128/JVI.79.22.14197-14206.2005  PMID: 16254355 
  30. Eis-Hübinger AM, Reber U, Edelmann A, Kalus U, Hofmann J. Parvovirus B19 genotype 2 in blood donations. Transfusion. 2014;54(6):1682-4.  https://doi.org/10.1111/trf.12591  PMID: 24911910 
  31. Grabarczyk P, Kalińska A, Kara M, Wieczorek R, Ejduk A, Sulkowska E, et al. Identification and characterization of acute infection with parvovirus B19 genotype 2 in immunocompromised patients in Poland. J Med Virol. 2011;83(1):142-9.  https://doi.org/10.1002/jmv.21947  PMID: 21108352 
  32. Norja P, Hokynar K, Aaltonen LM, Chen R, Ranki A, Partio EK, et al. Bioportfolio: lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue. Proc Natl Acad Sci USA. 2006;103(19):7450-3.  https://doi.org/10.1073/pnas.0602259103  PMID: 16651522 
  33. Schneider B, Höne A, Tolba RH, Fischer HP, Blümel J, Eis-Hübinger AM. Simultaneous persistence of multiple genome variants of human parvovirus B19. J Gen Virol. 2008;89(Pt 1):164-76.  https://doi.org/10.1099/vir.0.83053-0  PMID: 18089740 
  34. Parsyan A, Szmaragd C, Allain JP, Candotti D. Identification and genetic diversity of two human parvovirus B19 genotype 3 subtypes. J Gen Virol. 2007;88(Pt 2):428-31.  https://doi.org/10.1099/vir.0.82496-0  PMID: 17251559 
  35. Toan NL, Duechting A, Kremsner PG, Song LH, Ebinger M, Aberle S, et al. Phylogenetic analysis of human parvovirus B19, indicating two subgroups of genotype 1 in Vietnamese patients. J Gen Virol. 2006;87(Pt 10):2941-9.  https://doi.org/10.1099/vir.0.82037-0  PMID: 16963753 
Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Stefka+Krumova+Ivanova&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ivanova Stefka Krumova</a>, <a href="/search?value1=Zafira+Georgieva+Mihneva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mihneva Zafira Georgieva</a>, <a href="/search?value1=Andon+Krumov+Toshev&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Toshev Andon Krumov</a>, <a href="/search?value1=Valentina+Pavlova+Kovaleva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kovaleva Valentina Pavlova</a>, <a href="/search?value1=Lubena+Georgieva+Andonova&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Andonova Lubena Georgieva</a>, <a href="/search?value1=Claude+P+Muller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muller Claude P</a>, <a href="/search?value1=Judith+M+H%C3%BCbschen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hübschen Judith M</a></span>. Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013 . <a href="/content/ecdc">Euro Surveill.</a> 2016;21(4):pii=30116. <a href="https://doi.org/10.2807/1560-7917.ES.2016.21.4.30116" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2016.21.4.30116</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 30 Apr 2015;&nbsp;&nbsp; <span class="generated">Accepted</span>: 12 Nov 2015 </span> </p>
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