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Abstract

The present study aimed to determine the role of human parvovirus В19 (B19V) as an aetiological agent in measles and rubella negative fever/rash patients from Bulgaria between 2004 and 2013. A total of 1,266 sera from all over the country were tested for B19V IgM antibodies and all positives were further investigated by polymerase chain reaction (PCR). Overall, 280 sera (22%) were B19V IgM positive and 227 of these (81%) were also PCR positive. The highest number of IgM positives was found among five to nine year-old children (27%). Eight infected women gave birth to healthy children; one fetus was aborted with hydrops fetalis. Of the 55 genetic sequences obtained, 54 belonged to genotype 1a and one grouped as a genotype 2 outlier. Phylogenetic analysis of all available genotype 2 sequences covering the 994 nucleotide non-structural protein 1(NS1)/capsid viral protein 1 (VP1) unique region junction, showed that only one other sequence grouped with the outlier strain, forming a clearly distinct and well-supported cluster of genotype 2 (between-group genetic distance: 3.32%). In accordance with B19V nomenclature, this cluster may represent a new subgenotype 2b. The study showed that B19V infections may be falsely identified as rubella or measles in ca 22% of cases, emphasising the need for laboratory confirmation.

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/content/10.2807/1560-7917.ES.2016.21.4.30116
2016-01-28
2022-05-24
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2016.21.4.30116
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Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Stefka+Krumova+Ivanova&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ivanova Stefka Krumova</a>, <a href="/search?value1=Zafira+Georgieva+Mihneva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mihneva Zafira Georgieva</a>, <a href="/search?value1=Andon+Krumov+Toshev&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Toshev Andon Krumov</a>, <a href="/search?value1=Valentina+Pavlova+Kovaleva&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kovaleva Valentina Pavlova</a>, <a href="/search?value1=Lubena+Georgieva+Andonova&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Andonova Lubena Georgieva</a>, <a href="/search?value1=Claude+P+Muller&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Muller Claude P</a>, <a href="/search?value1=Judith+M+H%C3%BCbschen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hübschen Judith M</a></span>. Insights into epidemiology of human parvovirus B19 and detection of an unusual genotype 2 variant, Bulgaria, 2004 to 2013 . <a href="/content/ecdc">Euro Surveill.</a> 2016;21(4):pii=30116. <a href="https://doi.org/10.2807/1560-7917.ES.2016.21.4.30116" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2016.21.4.30116</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 30 Apr 2015;&nbsp;&nbsp; <span class="generated">Accepted</span>: 12 Nov 2015 </span> </p>
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