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To investigate the aetiology of the 2015 A(H1N1)pdm09 influenza outbreak in India, 1,083 nasopharyngeal swabs from suspect patients were screened for influenza A(H1N1)pdm09 in the state of Madhya Pradesh. Of 412 positive specimens, six were further characterised by phylogenetic analysis of haemagglutinin (HA) sequences revealing that they belonged to genogroup 6B. A new mutation (E164G) was observed in HA2 of two sequences. Neuraminidase genes in two of 12 isolates from fatal cases on prior oseltamivir treatment harboured the H275Y mutation.


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  1. Cousins S. Death toll from swine flu in India exceeds 2500. BMJ. 2015;351:h4966.  https://doi.org/10.1136/bmj.h4966  PMID: 26384378 
  2. Pandemic influenza (h1n1) 2009. CD Alert. 2009; 13(2). Available from: http://www.ncdc.gov.in/writereaddata/linkimages/Aug-Sep_092813460594.pdf
  3. World Health Organization (WHO), Centers for Disease Control and Prevention (CDC). CDC protocol of real-time RTPCR for swine influenza A(H1N1). Geneva: WHO; 2009. [Accessed 15 Mar 2015]. Available from: http://www.who.int/csr/resources/publications/swineflu/CDCrealtimeRTPCRprotocol_20090428.pdf
  4. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28(10):2731-9.  https://doi.org/10.1093/molbev/msr121  PMID: 21546353 
  5. Nukiwa N, Suzuki A, Furuse Y, Shimabukuro K, Odagiri T, Khandaker I, et al. Simplified screening method for detecting oseltamivir resistant pandemic influenza A (H1N1) 2009 virus by a RT-PCR/restriction fragment length polymorphism assay. J Virol Methods. 2010;170(1-2):165-8.  https://doi.org/10.1016/j.jviromet.2010.09.005  PMID: 20833206 
  6. Jiménez-Jorge S, Pozo F, de Mateo S, Delgado-Sanz C, Casas I, García-Cenoz M, et al. Spanish Influenza Sentinel Surveillance System (SISS). Influenza vaccine effectiveness in Spain 2013/14: subtype-specific early estimates using the cycEVA study. Euro Surveill. 2014;19(9):20727.  https://doi.org/10.2807/1560-7917.ES2014.19.9.20727  PMID: 24626206 
  7. European Centre of Disease Prevention and Control (ECDC). Surveillance Report. Influenza virus characterization. Summary Europe, December 2013. Stockholm: ECDC; 2013. Available from: http://www.ecdc.europa.eu/en/publications/Publications/influenza-virus-characterisation-dec-2013.pdf
  8. Castelán-Vega JA, Magaña-Hernández A, Jiménez-Alberto A, Ribas-Aparicio RM. The hemagglutinin of the influenza A(H1N1)pdm09 is mutating towards stability. Adv Appl Bioinform Chem. 2014;7:37-44.  https://doi.org/10.2147/AABC.S68934  PMID: 25328411 
  9. Dakhave M, Khirwale A, Patil K, Kadam A, Potdar V. Whole-genome sequence analysis of postpandemic influenza A(H1N1)pdm09 virus isolates from India. Genome Announc. 2013;1(5):e00727-13.  https://doi.org/10.1128/genomeA.00727-13  PMID: 24051317 
  10. European Centre of Disease Prevention and Control (ECDC). Surveillance Report. Influenza virus characterization, summary Europe, September 2015. Stockholm: ECDC; 2015. Available from: http://ecdc.europa.eu/en/publications/Publications/influenza-virus-characterisation-september-2015.pdf
  11. Deem MW, Pan K. The epitope regions of H1-subtype influenza A, with application to vaccine efficacy. Protein Eng Des Sel. 2009;22(9):543-6.  https://doi.org/10.1093/protein/gzp027  PMID: 19578121 
  12. Cases of Influenza A H1N1 (Swine Flu) – State/UT- wise, Year- wise for 2009, 2010, 2011 and 2012; Ministry of Health & Family Welfare, Govt. of India. Available from: http://mohfw.nic.in/showfile.php?lid=2121
  13. Broor S, Krishnan A, Roy DS, Dhakad S, Kaushik S, Mir MA, et al. Dynamic patterns of circulating seasonal and pandemic A(H1N1)pdm09 influenza viruses from 2007-2010 in and around Delhi, India. PLoS ONE. 2012;7(1):e29129.  https://doi.org/10.1371/journal.pone.0029129  PMID: 22235265 
  14. Tharakaraman K, Sasisekharan R. Influenza surveillance: 2014-2015 H1N1 "swine"-derived influenza viruses from India. Cell Host Microbe. 2015;17(3):279-82.  https://doi.org/10.1016/j.chom.2015.02.019  PMID: 25766285 
  15. Neumann G, Noda T, Kawaoka Y. Emergence and pandemic potential of swine-origin H1N1 influenza virus. Nature. 2009;459(7249):931-9.  https://doi.org/10.1038/nature08157  PMID: 19525932 
  16. Ortiz JR, Sotomayor V, Uez OC, Oliva O, Bettels D, McCarron M, et al. Strategy to enhance influenza surveillance worldwide. Emerg Infect Dis. 2009;15(8):1271-8.  https://doi.org/10.3201/eid1508.081422  PMID: 19751590 

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