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Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
- Tansy Peters1 , Sophie Bertrand2 , Jonas T Björkman3 , Lin T Brandal4 , Derek J Brown5 , Tímea Erdõsi6 , Max Heck7 , Salha Ibrahem8 , Karin Johansson9 , Christian Kornschober10 , Saara M Kotila9 , Simon Le Hello11 , Taru Lienemann8 , Wesley Mattheus2 , Eva Møller Nielsen3 , Catherine Ragimbeau12 , Jillian Rumore13 , Ashley Sabol14 , Mia Torpdahl3 , Eija Trees14 , Alma Tuohy15 , Elizabeth de Pinna1
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View Affiliations Hide AffiliationsAffiliations: 1 Public Health England, London, United Kingdom 2 Scientific Institute of Public Health, Brussels, Belgium 3 Statens Serum Institut, Copenhagen, Denmark 4 Norwegian Institute of Public Health, Oslo, Norway 5 Scottish Microbiology Reference Laboratories, Glasgow, United Kingdom 6 National Center for Epidemiology, Budapest, Hungary 7 National Institute for Public Health and the Environment, Bilthoven, The Netherlands 8 National Institute for Health and Welfare, Helsinki, Finland 9 European Centre for Disease Prevention and Control (ECDC), Solna, Sweden 10 Austrian Agency for Health and Food Safety, Graz, Austria 11 Institut Pasteur, Paris, France 12 Laboratoire National de Santé, Dudelange, Luxembourg 13 Public Health Agency of Canada, Winnipeg, Canada 14 Centers for Disease Control and Prevention, Atlanta, USA 15 University Hospital Galway, Galway, IrelandSaara M Kotilasaara.kotila ecdc.europa.eu
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Citation style for this article: Peters Tansy, Bertrand Sophie, Björkman Jonas T, Brandal Lin T, Brown Derek J, Erdõsi Tímea, Heck Max, Ibrahem Salha, Johansson Karin, Kornschober Christian, Kotila Saara M, Le Hello Simon, Lienemann Taru, Mattheus Wesley, Nielsen Eva Møller, Ragimbeau Catherine, Rumore Jillian, Sabol Ashley, Torpdahl Mia, Trees Eija, Tuohy Alma, de Pinna Elizabeth. Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015. Euro Surveill. 2017;22(9):pii=30477. https://doi.org/10.2807/1560-7917.ES.2017.22.9.30477 Received: 14 Jan 2016; Accepted: 10 May 2016
Abstract
Multilocus variable-number tandem repeat analysis (MLVA) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. Salmonella enterica serovar Enteritidis is the most common Salmonella serovar associated with human salmonellosis in the European Union/European Economic Area and North America. Fourteen laboratories from 13 countries in Europe and North America participated in a validation study for MLVA of S. Enteritidis targeting five loci. Following normalisation of fragment sizes using a set of reference strains, a blinded set of 24 strains with known allele sizes was analysed by each participant. The S. Enteritidis 5-loci MLVA protocol was shown to produce internationally comparable results as more than 90% of the participants reported less than 5% discrepant MLVA profiles. All 14 participating laboratories performed well, even those where experience with this typing method was limited. The raw fragment length data were consistent throughout, and the inter-laboratory validation helped to standardise the conversion of raw data to repeat numbers with at least two countries updating their internal procedures. However, differences in assigned MLVA profiles remain between well-established protocols and should be taken into account when exchanging data.

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