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Abstract

Multilocus variable-number tandem repeat analysis (MLVA) is a rapid and reproducible typing method that is an important tool for investigation, as well as detection, of national and multinational outbreaks of a range of food-borne pathogens. S serovar Enteritidis is the most common serovar associated with human salmonellosis in the European Union/European Economic Area and North America. Fourteen laboratories from 13 countries in Europe and North America participated in a validation study for MLVA of Enteritidis targeting five loci. Following normalisation of fragment sizes using a set of reference strains, a blinded set of 24 strains with known allele sizes was analysed by each participant. The Enteritidis 5-loci MLVA protocol was shown to produce internationally comparable results as more than 90% of the participants reported less than 5% discrepant MLVA profiles. All 14 participating laboratories performed well, even those where experience with this typing method was limited. The raw fragment length data were consistent throughout, and the inter-laboratory validation helped to standardise the conversion of raw data to repeat numbers with at least two countries updating their internal procedures. However, differences in assigned MLVA profiles remain between well-established protocols and should be taken into account when exchanging data.

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/content/10.2807/1560-7917.ES.2017.22.9.30477
2017-03-02
2018-12-19
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2017.22.9.30477
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Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Tansy+Peters&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Peters Tansy</a>, <a href="/search?value1=Sophie+Bertrand&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Bertrand Sophie</a>, <a href="/search?value1=Jonas+T+Bj%C3%B6rkman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Björkman Jonas T</a>, <a href="/search?value1=Lin+T+Brandal&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brandal Lin T</a>, <a href="/search?value1=Derek+J+Brown&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brown Derek J</a>, <a href="/search?value1=T%C3%ADmea+Erd%C3%B5si&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Erdõsi Tímea</a>, <a href="/search?value1=Max+Heck&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Heck Max</a>, <a href="/search?value1=Salha+Ibrahem&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ibrahem Salha</a>, <a href="/search?value1=Karin+Johansson&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Johansson Karin</a>, <a href="/search?value1=Christian+Kornschober&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kornschober Christian</a>, <a href="/search?value1=Saara+M+Kotila&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Kotila Saara M</a>, <a href="/search?value1=Simon+Le+Hello&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Le Hello Simon</a>, <a href="/search?value1=Taru+Lienemann&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lienemann Taru</a>, <a href="/search?value1=Wesley+Mattheus&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mattheus Wesley</a>, <a href="/search?value1=Eva+M%C3%B8ller+Nielsen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nielsen Eva Møller</a>, <a href="/search?value1=Catherine+Ragimbeau&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ragimbeau Catherine</a>, <a href="/search?value1=Jillian+Rumore&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rumore Jillian</a>, <a href="/search?value1=Ashley+Sabol&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sabol Ashley</a>, <a href="/search?value1=Mia+Torpdahl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Torpdahl Mia</a>, <a href="/search?value1=Eija+Trees&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Trees Eija</a>, <a href="/search?value1=Alma+Tuohy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tuohy Alma</a>, <a href="/search?value1=Elizabeth+de+Pinna&option1=author&noRedirect=true" class="nonDisambigAuthorLink">de Pinna Elizabeth</a></span>. Multi-laboratory validation study of multilocus variable-number tandem repeat analysis (MLVA) for Salmonella enterica serovar Enteritidis, 2015. <a href="/content/ecdc">Euro Surveill.</a> 2017;22(9):pii=30477. <a href="https://doi.org/10.2807/1560-7917.ES.2017.22.9.30477" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2017.22.9.30477</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 Jan 2016;&nbsp;&nbsp; <span class="generated">Accepted</span>: 10 May 2016 </span> </p>
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