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Surveillance and outbreak report Open Access
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Abstract

In May 2016, two cases of tick-borne encephalitis (TBE) were confirmed by serology (positive IgM and IgG antibodies against TBE virus (TBEV) in serum), with a possible link to raw milk and cheese from a goat farm in a region in Baden-Württemberg, Germany not previously known as TBE-endemic. The outbreak investigation identified 32 consumers of goat dairy products (29 consumers, one farm employee, two owners) of whom none had IgM antibodies against TBEV 3–8 weeks after consumption. Of the 27 notified TBE cases in the State, none reported consumption of raw goat milk or cheese from the suspected farm. Five of 22 cheese samples from 18 different batches were RT-qPCR-positive for TBEV -genome, and two of the five samples were confirmed by virus isolation, indicating viability of TBEV in the cheese. Nine of the 45 goats had neutralising TBEV antibodies, two of them with a high titre indicating recent infection. One of 412 was RT-qPCR-positive, and sequencing of the E gene from nucleic acid extracted from the tick confirmed TBEV. Phylogenetic analyses of tick and cheese isolates showed 100% amino acid homology in the E gene and a close relation to TBEV strains from Switzerland and Austria.

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/content/10.2807/1560-7917.ES.2018.23.15.17-00336
2018-04-12
2024-12-02
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2018.23.15.17-00336
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References

  1. Lindquist L, Vapalahti O. Tick-borne encephalitis. Lancet. 2008;371(9627):1861-71.  https://doi.org/10.1016/S0140-6736(08)60800-4  PMID: 18514730 
  2. Bogovic P, Strle F. Tick-borne encephalitis: A review of epidemiology, clinical characteristics, and management. World J Clin Cases. 2015;3(5):430-41.  https://doi.org/10.12998/wjcc.v3.i5.430  PMID: 25984517 
  3. Klaus C, Hoffmann D, Hoffmann B, Beer M. Frühsommer-Meningoenzephalitis-Virus-Infektionen bei Tieren – Klinik, Diagnostik und epidemiologische Bedeutung.[Tick-borne encephalitis infections in animals – clinical symptoms, diagnostics and epidemiological relevance]. Berl Munch Tierarztl Wochenschr. 2016; advance online publication. German.  https://doi.org/10.2376/0005-9366-16062 
  4. Klaus C, Beer M, Saier R, Schau U, Moog U, Hoffmann B, et al. Goats and sheep as sentinels for tick-borne encephalitis (TBE) virus--epidemiological studies in areas endemic and non-endemic for TBE virus in Germany. Ticks Tick Borne Dis. 2012;3(1):27-37.  https://doi.org/10.1016/j.ttbdis.2011.09.011  PMID: 22309856 
  5. Gresíková M, Sekeyová M, Stúpalová S, Necas S. Sheep milk-borne epidemic of tick-borne encephalitis in Slovakia. Intervirology. 1975;5(1-2):57-61.  https://doi.org/10.1159/000149880  PMID: 1237478 
  6. Amicizia D, Domnich A, Panatto D, Lai PL, Cristina ML, Avio U, et al. Epidemiology of tick-borne encephalitis (TBE) in Europe and its prevention by available vaccines. Hum Vaccin Immunother. 2013;9(5):1163-71.  https://doi.org/10.4161/hv.23802  PMID: 23377671 
  7. Gresikova M, Rehacek J. Isolierung des Zeckenenzephalitisvirus aus Blut und Milch von Haustieren (Schaf und Kuh) nach Infektion durch Zecken der Gattung Ixodes ricinus L. [Isolation of the tick encephalitis virus from blood ad milk of domestic animals (sheep and cow) after infection by ticks of the family Ixodes ricinus L.]. Arch Gesamte Virusforsch. 1959;9(3):360-4. German.  https://doi.org/10.1007/BF01248828  PMID: 13829212 
  8. Gresikova M. [The transmission of the virus of the Czechoslovak tick encephalitis by goat milk]. In: Zeckenenzephalitis in Europa [Tick-borne encephalitis in Europe]. Libikova H, editor. Abhandlungen der Deutschen Akademie der Wissenschaften zu Berlin. Akademieverlag Berlin. Vol 1960 (2):121-2.
  9. Kríz B, Benes C, Daniel M. Alimentary transmission of tick-borne encephalitis in the Czech Republic (1997-2008). Epidemiol Mikrobiol Imunol. 2009;58(2):98-103. PMID: 19526924 
  10. Cisak E, Wójcik-Fatla A, Zając V, Sroka J, Buczek A, Dutkiewicz J. Prevalence of tick-borne encephalitis virus (TBEV) in samples of raw milk taken randomly from cows, goats and sheep in eastern Poland. Ann Agric Environ Med. 2010;17(2):283-6. PMID: 21186771 
  11. Caini S, Szomor K, Ferenczi E, Szekelyne Gaspar A, Csohan A, Krisztalovics K, et al. Tick-borne encephalitis transmitted by unpasteurised cow milk in western Hungary, September to October 2011. Euro Surveill. 2012;17(12):20128. PMID: 22490310 
  12. Hudopisk N, Korva M, Janet E, Simetinger M, Grgič-Vitek M, Gubenšek J, et al. Tick-borne encephalitis associated with consumption of raw goat milk, Slovenia, 2012. Emerg Infect Dis. 2013;19(5):806-8.  https://doi.org/10.3201/eid1905.121442  PMID: 23697658 
  13. Markovinović L, Kosanović Ličina ML, Tešić V, Vojvodić D, Vladušić Lucić I, Kniewald T, et al. An outbreak of tick-borne encephalitis associated with raw goat milk and cheese consumption, Croatia, 2015. Infection. 2016;44(5):661-5.  https://doi.org/10.1007/s15010-016-0917-8  PMID: 27364148 
  14. Holzmann H, Aberle SW, Stiasny K, Werner P, Mischak A, Zainer B, et al. Tick-borne encephalitis from eating goat cheese in a mountain region of Austria. Emerg Infect Dis. 2009;15(10):1671-3.  https://doi.org/10.3201/eid1510.090743  PMID: 19861072 
  15. Robert Koch Institut (RKI). SurvStat@RKI 2.0. Berlin: RKI. [Accessed: 2 May 2017]. https://survstat.rki.de/
  16. Schwaiger M, Cassinotti P. Development of a quantitative real-time RT-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA. J Clin Virol. 2003;27(2):136-45.  https://doi.org/10.1016/S1386-6532(02)00168-3  PMID: 12829035 
  17. Kupča AM, Essbauer S, Zoeller G, de Mendonça PG, Brey R, Rinder M, et al. Isolation and molecular characterization of a tick-borne encephalitis virus strain from a new tick-borne encephalitis focus with severe cases in Bavaria, Germany. Ticks Tick Borne Dis. 2010;1(1):44-51.  https://doi.org/10.1016/j.ttbdis.2009.11.002  PMID: 21771510 
  18. Klaus C, Beer M, Saier R, Schubert H, Bischoff S, Süss J. Evaluation of serological tests for detecting tick-borne encephalitis virus (TBEV) antibodies in animals. Berl Munch Tierarztl Wochenschr. 2011;124(11-12):443-9. PMID: 22191165 
  19. Gäumann R, Mühlemann K, Strasser M, Beuret CM. High-throughput procedure for tick surveys of tick-borne encephalitis virus and its application in a national surveillance study in Switzerland. Appl Environ Microbiol. 2010;76(13):4241-9.  https://doi.org/10.1128/AEM.00391-10  PMID: 20453126 
  20. Rieger MA, Nübling M, Kaiser R, Tiller FW, Hofmann F. FSME-Infektionen durch Rohmilch--welche Rolle spielt dieser Infektionsweg? Untersuchungen aus dem südwestdeutschen FSME-Endemiegebiet. Gesundheitswesen. 1998;60(6):348-56. PMID: 9697358 
  21. Dobler G, Bestehorn M, Antwerpen M, Överby-Wernstedt A. Complete genome sequence of a low-virulence tick-borne encephalitis virus strain. Genome Announc. 2016;4(5):e01145-16.  https://doi.org/10.1128/genomeA.01145-16  PMID: 27795275 
  22. Salat J, Slosarkova S, Ruzek D, Hunady M. Tick-borne encephalitis: The risk of alimentary infection of small ruminants in the Czech Republic. Poster presented at: 9th Conference on Ticks and Tick-borne Pathogens; 27 Aug-1 Sep 2017; Cairns, Australia.
  23. Saier R, Hinrichs J. FSME-Antikörper-Vorkommen bei Ziegen aus Baden-Württemberg und Untersuchungen zur thermischen Stabilität von FSME-Erregern in Milch [Occurrence of TBE antibodies in goats from Baden-Württemberg and studies on the thermal stability of TBE viruses in milk]. German. Presented at: 2. Süddeutscher Zeckenkongress; 17-18 Mar 2014; Stuttgart, Germany.
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