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Abstract

In Italy, B and C are the predominant serogroups among meningococci causing invasive diseases. Nevertheless, in the period from 2013 to 2016, an increase in serogroup W (MenW) was observed. This study intends to define the main characteristics of 63 MenW isolates responsible of invasive meningococcal disease (IMD) in Italy from 2000 to 2016. We performed whole genome sequencing on bacterial isolates or single gene sequencing on culture-negative samples to evaluate molecular heterogeneity. Our main finding was the cocirculation of the Hajj and the South American sublineages belonging to MenW/clonal complex (cc)11, which gradually surpassed the MenW/cc22 in Italy. All MenW/cc11 isolates were fully susceptible to cefotaxime, ceftriaxone, ciprofloxacin, penicillin G and rifampicin. We identified the full-length NadA protein variant 2/3, present in all the MenW/cc11. We also identified the fHbp variant 1, which we found exclusively in the MenW/cc11/Hajj sublineage. Concern about the epidemic potential of MenW/cc11 has increased worldwide since the year 2000. Continued surveillance, supported by genomic characterisation, allows high-resolution tracking of pathogen dissemination and the detection of epidemic-associated strains.

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/content/10.2807/1560-7917.ES.2019.24.4.1800183
2019-01-24
2019-02-18
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.4.1800183
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References

  1. Mayer LW, Reeves MW, Al-Hamdan N, Sacchi CT, Taha MK, Ajello GW, et al. Outbreak of W135 meningococcal disease in 2000: not emergence of a new W135 strain but clonal expansion within the electophoretic type-37 complex. J Infect Dis. 2002;185(11):1596-605.  https://doi.org/10.1086/340414  PMID: 12023765 
  2. Abad R, López EL, Debbag R, Vázquez JA. Serogroup W meningococcal disease: global spread and current affect on the Southern Cone in Latin America. Epidemiol Infect. 2014;142(12):2461-70.  https://doi.org/10.1017/S0950268814001149  PMID: 24831052 
  3. Lemos AP, Harrison LH, Lenser M, Sacchi CT. Phenotypic and molecular characterization of invasive serogroup W135 Neisseria meningitidis strains from 1990 to 2005 in Brazil. J Infect. 2010;60(3):209-17.  https://doi.org/10.1016/j.jinf.2009.11.014  PMID: 20056121 
  4. Mustapha MM, Marsh JW, Krauland MG, Fernandez JO, de Lemos AP, Dunning Hotopp JC, et al. Genomic epidemiology of hypervirulent serogroup W, ST-11 Neisseria meningitidis. EBioMedicine. 2015;2(10):1447-55.  https://doi.org/10.1016/j.ebiom.2015.09.007  PMID: 26629539 
  5. Lucidarme J, Hill DM, Bratcher HB, Gray SJ, du Plessis M, Tsang RSW, et al. Genomic resolution of an aggressive, widespread, diverse and expanding meningococcal serogroup B, C and W lineage. J Infect. 2015;71(5):544-52.  https://doi.org/10.1016/j.jinf.2015.07.007  PMID: 26226598 
  6. Eriksson L, Hedberg ST, Jacobsson S, Fredlund H, Mölling P, Stenmark B. Whole genome sequencing of the emerging invasive Neisseria meningitidis serogroup W in Sweden. J Clin Microbiol. 2018;56(4):e01409-17.  https://doi.org/10.1128/JCM.01409-17  PMID: 29321195 
  7. Knol MJ, Hahné SJM, Lucidarme J, Campbell H, de Melker HE, Gray SJ, et al. Temporal associations between national outbreaks of meningococcal serogroup W and C disease in the Netherlands and England: an observational cohort study. Lancet Public Health. 2017;2(10):e473-82.  https://doi.org/10.1016/S2468-2667(17)30157-3  PMID: 29253430 
  8. Mustapha MM, Marsh JW, Harrison LH. Global epidemiology of capsular group W meningococcal disease (1970-2015): Multifocal emergence and persistence of hypervirulent sequence type (ST)-11 clonal complex. Vaccine. 2016;34(13):1515-23.  https://doi.org/10.1016/j.vaccine.2016.02.014  PMID: 26876439 
  9. Lucidarme J, Scott KJ, Ure R, Smith A, Lindsay D, Stenmark B, et al. An international invasive meningococcal disease outbreak due to a novel and rapidly expanding serogroup W strain, Scotland and Sweden, July to August 2015. Euro Surveill. 2016;21(45):30395.  https://doi.org/10.2807/1560-7917.ES.2016.21.45.30395  PMID: 27918265 
  10. von Gottberg A, du Plessis M, Cohen C, Prentice E, Schrag S, de Gouveia L, et al. Emergence of endemic serogroup W135 meningococcal disease associated with a high mortality rate in South Africa. Clin Infect Dis. 2008;46(3):377-86.  https://doi.org/10.1086/525260  PMID: 18181736 
  11. Parent du Chatelet I, Barboza P, Taha MK. W135 invasive meningococcal infections imported from Sub-Saharan Africa to France, January to April 2012. Euro Surveill. 2012;17(21):20181. PMID: 22687826 
  12. Taha MK, Kacou-N’douba A, Hong E, Deghmane AE, Giorgini D, Okpo SL, et al. Travel-related Neisseria meningitidis serogroup W135 infection, France. Emerg Infect Dis. 2013;19(6):1030-2.  https://doi.org/10.3201/eid1906.120515  PMID: 23735310 
  13. Fazio C, Neri A, Renna G, Vacca P, Antonetti R, Barbui AM, et al. Persistent occurrence of serogroup Y/sequence type (ST)-23 complex invasive meningococcal disease among patients aged five to 14 years, Italy, 2007 to 2013. Euro Surveill. 2015;20(45):30061.  https://doi.org/10.2807/1560-7917.ES.2015.20.45.30061  PMID: 26606870 
  14. Mastrantonio P, Stefanelli P, Fazio C, Sofia T, Neri A, La Rosa G, et al. Serotype distribution, antibiotic susceptibility, and genetic relatedness of Neisseria meningitidis strains recently isolated in Italy. Clin Infect Dis. 2003;36(4):422-8.  https://doi.org/10.1086/346154  PMID: 12567299 
  15. Zhu H, Wang Q, Wen L, Xu J, Shao Z, Chen M, et al. Development of a multiplex PCR assay for detection and genogrouping of Neisseria meningitidis. J Clin Microbiol. 2012;50(1):46-51.  https://doi.org/10.1128/JCM.00918-11  PMID: 22090406 
  16. The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. Version 8.1, 2018. Växjö: EUCAST; 2018. Available from: http://www.eucast.org/clinical_breakpoints/
  17. Andrews S. FastQC A quality control tool for high throughput sequence data. Cambridge: Babraham Institute; 2010. Available from: http://www.bioinformatics.babraham.ac.uk/projects/fastqc
  18. Joshi NA, Fass JN. Sickle: A sliding-window, adaptive, quality-based trimming tool for FastQ files using quality (Version 1.33). 2011. Available from: https://github.com/najoshi/sickle
  19. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ, Birol I. ABySS: a parallel assembler for short read sequence data. Genome Res. 2009;19(6):1117-23.  https://doi.org/10.1101/gr.089532.108  PMID: 19251739 
  20. Jolley KA, Maiden MC. BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics. 2010;11(1):595.  https://doi.org/10.1186/1471-2105-11-595  PMID: 21143983 
  21. Bratcher HB, Corton C, Jolley KA, Parkhill J, Maiden MC. A gene-by-gene population genomics platform: de novo assembly, annotation and genealogical analysis of 108 representative Neisseria meningitidis genomes. BMC Genomics. 2014;15(1):1138.  https://doi.org/10.1186/1471-2164-15-1138  PMID: 25523208 
  22. Huson DH, Bryant D. Application of phylogenetic networks in evolutionary studies. Mol Biol Evol. 2006;23(2):254-67.  https://doi.org/10.1093/molbev/msj030  PMID: 16221896 
  23. Stefanelli P, Fazio C, Neri A, Rezza G, Severoni S, Vacca P, et al. Imported and Indigenous cases of Invasive Meningocococcal Disease W:P1.5,2:F1-1: ST-11 in migrants’ reception centers. Italy, June-November 2014. Adv Exp Med Biol. 2016;897:81-3.  https://doi.org/10.1007/5584_2015_5006  PMID: 26563305 
  24. Campbell H, Parikh SR, Borrow R, Kaczmarski E, Ramsay ME, Ladhani SN. Presentation with gastrointestinal symptoms and high case fatality associated with group W meningococcal disease (MenW) in teenagers, England, July 2015 to January 2016. Euro Surveill. 2016;21(12):30175.  https://doi.org/10.2807/1560-7917.ES.2016.21.12.30175  PMID: 27035055 
  25. Mowlaboccus S, Jolley KA, Bray JE, Pang S, Lee YT, Bew JD, et al. Clonal expansion of new penicillin-resistant clade of Neisseria meningitidis serogroup W clonal complex 11, Australia. Emerg Infect Dis. 2017;23(8):1364-7.  https://doi.org/10.3201/eid2308.170259  PMID: 28609259 
  26. Hong E, Barret AS, Terrade A, Denizon M, Antona D, Aouiti-Trabelsi M, et al. Clonal replacement and expansion among invasive meningococcal isolates of serogroup W in France. J Infect. 2018;76(2):149-58.  https://doi.org/10.1016/j.jinf.2017.10.015  PMID: 29132919 
  27. Piano Nazionale Prevenzione Vaccinale PNPV 2017-2019 [National Plan for Vaccine Prevention NPVP 2017-2019 ] Rome: Ministero della Salute, 2017. Italian. Available from: http://www.salute.gov.it/imgs/C_17_pubblicazioni_2571_allegato.pdf
  28. Ladhani SN, Giuliani MM, Biolchi A, Pizza M, Beebeejaun K, Lucidarme J, et al. Effectiveness of meningococcal B vaccine against endemic hypervirulent Neisseria meningitidis W strain, England. Emerg Infect Dis. 2016;22(2):309-11.  https://doi.org/10.3201/eid2202.150369  PMID: 26811872 
  29. Hong E, Giuliani MM, Deghmane AE, Comanducci M, Brunelli B, Dull P, et al. Could the multicomponent meningococcal serogroup B vaccine (4CMenB) control Neisseria meningitidis capsular group X outbreaks in Africa? Vaccine. 2013;31(7):1113-6.  https://doi.org/10.1016/j.vaccine.2012.12.022  PMID: 23261039 
  30. Vogel U, Taha MK, Vazquez JA, Findlow J, Claus H, Stefanelli P, et al. Predicted strain coverage of a meningococcal multicomponent vaccine (4CMenB) in Europe: a qualitative and quantitative assessment. Lancet Infect Dis. 2013;13(5):416-25.  https://doi.org/10.1016/S1473-3099(13)70006-9  PMID: 23414709 
  31. Trotter CL, Findlow H, Borrow R. Seroprevalence of serum bactericidal antibodies against group W135 and Y meningococci in England in 2009. Clin Vaccine Immunol. 2012;19(2):219-22.  https://doi.org/10.1128/CVI.05515-11  PMID: 22190393 
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