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Abstract

Introduction

During summer 2016, Norway observed an increase in subsp. serovar Chester cases among travellers to Greece.

Aim

Our aim was to investigate genetic relatedness of Chester for surveillance and outbreak detection by core genome multilocus sequence typing (cgMLST) and compare the results to genome mapping.

Methods

We included Chester isolates from 51 cases of salmonellosis between 2000 and 2016. Paired-end sequencing (2 × 250 bp) was performed on Illumina MiSeq. Genetic relatedness by cgMLST for subsp. including 3,002 genes and seven housekeeping genes, was compared by reference genome mapping with CSI Phylogeny version 1.4 and conventional MLST.

Results

Confirmed travel history was available for 80% of included cases, to Europe (n = 13), Asia (n = 12) and Africa (n = 16). Isolates were distributed into four phylogenetic clusters corresponding to geographical regions. Sequence type (ST) ST411 and a single-locus variant ST5260 (n = 17) were primarily acquired in southern Europe, ST1954 (n = 15) in Africa, ST343 (n = 11) and ST2063 (n = 8) primarily in Asia. Part of the European cluster was further divided into a Greek (n = 10) and a Cypriot (n = 4) cluster. All isolates in the African cluster displayed resistance to ≥ 1 class of antimicrobials, while resistance was rare in the other clusters.

Conclusion

Whole genome sequencing of Chester in Norway showed four geographically distinct clusters, with a possible outbreak occurring during summer 2016 related to Greece. We recommend public health institutes to implement cgMLST-based real-time surveillance for early and accurate detection of future outbreaks and further development of cluster cut-offs.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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2019-01-24
2024-04-25
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.4.1800186
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Whole genome sequencing of Salmonella Chester reveals geographically distinct clusters, Norway, 2000 to 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Lotta+Siira&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Siira Lotta</a>, <a href="/search?value1=Umaer+Naseer&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Naseer Umaer</a>, <a href="/search?value1=Kristian+Alfsnes&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Alfsnes Kristian</a>, <a href="/search?value1=Nils+Olav+Hermansen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hermansen Nils Olav</a>, <a href="/search?value1=Heidi+Lange&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lange Heidi</a>, <a href="/search?value1=Lin+T+Brandal&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Brandal Lin T</a></span>. Whole genome sequencing of Salmonella Chester reveals geographically distinct clusters, Norway, 2000 to 2016. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(4):pii=1800186. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.4.1800186" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.4.1800186</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 13 Apr 2018;&nbsp;&nbsp; <span class="generated">Accepted</span>: 06 Nov 2018 </span> </p>
/content/10.2807/1560-7917.ES.2019.24.4.1800186
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