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Abstract

Background

Middle East respiratory syndrome coronavirus (MERS-CoV) remains a major concern for global public health. Dromedaries are the source of human zoonotic infection. MERS-CoV is enzootic among dromedaries on the Arabian Peninsula, the Middle East and in Africa. Over 70% of infected dromedaries are found in Africa. However, all known zoonotic cases of MERS have occurred in the Arabian Peninsula with none being reported in Africa.

Aim

We aimed to investigate serological evidence of MERS-CoV infection in humans living in camel-herding areas in Morocco to provide insights on whether zoonotic transmission is taking place.

Methods

We carried out a cross sectional seroprevalence study from November 2017 through January 2018. We adapted a generic World Health Organization MERS-CoV questionnaire and protocol to assess demographic and risk factors of infection among a presumed high-risk population. ELISA, MERS-CoV spike pseudoparticle neutralisation tests (ppNT) and plaque neutralisation tests (PRNT) were used to assess MERS-CoV seropositivity.

Results

Serum samples were collected from camel slaughterhouse workers (n = 137), camel herders (n = 156) and individuals of the general population without occupational contact with camels but living in camel herding areas (n = 186). MERS-CoV neutralising antibodies with ≥ 90% reduction of plaque numbers were detected in two (1.5%) slaughterhouse workers, none of the camel herders and one individual from the general population (0.5%).

Conclusions

This study provides evidence of zoonotic transmission of MERS-CoV in Morocco in people who have direct or indirect exposure to dromedary camels.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
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/content/10.2807/1560-7917.ES.2019.24.48.1900244
2019-11-28
2020-01-21
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2019.24.48.1900244
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Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Anass+Abbad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Abbad Anass</a>, <a href="/search?value1=Ranawaka+APM+Perera&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Perera Ranawaka APM</a>, <a href="/search?value1=Latifa+Anga&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anga Latifa</a>, <a href="/search?value1=Abdellah+Faouzi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Faouzi Abdellah</a>, <a href="/search?value1=Nhu+Nguyen+Tran+Minh&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Minh Nhu Nguyen Tran</a>, <a href="/search?value1=Sk+Md+Mamunur+Rahman+Malik&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Malik Sk Md Mamunur Rahman</a>, <a href="/search?value1=Nadia+Iounes&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Iounes Nadia</a>, <a href="/search?value1=Abderrahmane+Maaroufi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Maaroufi Abderrahmane</a>, <a href="/search?value1=Maria+D+Van+Kerkhove&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Van Kerkhove Maria D</a>, <a href="/search?value1=Malik+Peiris&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Peiris Malik</a>, <a href="/search?value1=Jalal+Nourlil&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Nourlil Jalal</a></span>. Middle East respiratory syndrome coronavirus (MERS-CoV) neutralising antibodies in a high-risk human population, Morocco, November 2017 to January 2018. <a href="/content/ecdc">Euro Surveill.</a> 2019;24(48):pii=1900244. <a href="https://doi.org/10.2807/1560-7917.ES.2019.24.48.1900244" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2019.24.48.1900244</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 14 Apr 2019;&nbsp;&nbsp; <span class="generated">Accepted</span>: 06 Oct 2019 </span> </p>
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