1887
Rapid communication Open Access
Like 0

Abstract

Annually, increasing numbers of OXA-244-producing in 13 German federal states prompted us to initiate an outbreak investigation. Whole genome sequencing revealed that among 148 isolates analysed, most belonged to sequence type 38 with 62 isolates forming a genetically distinct cluster. Although no epidemiological link could be identified between cases, ongoing investigations suggest non-healthcare associated transmission. A screening-PCR was developed facilitating early detection of ST38 cluster isolates to identify the source and transmission route.

Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2020.25.25.2000923
2020-06-25
2020-09-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.25.2000923
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/25/25/eurosurv-25-25-1.html?itemId=/content/10.2807/1560-7917.ES.2020.25.25.2000923&mimeType=html&fmt=ahah

References

  1. European Centre for Disease Prevention and Control (ECDC). Rapid risk assessment: Increase in OXA-244-producing Escherichia coli in the European Union/European Economic Area and the UK since 2013. ECDC: Stockholm; 18 Feb 2020. Available from: https://www.ecdc.europa.eu/en/publications-data/rapid-risk-assessment-increase-oxa-244-producing-escherichia-coli-eu-eea
  2. Hans JB, Kramer R, Kremer K, Pfennigwerth N, Haller S, Reuss A, et al. Ongoing outbreak of OXA-244 carbapenemase-producing Enterobacterales in Germany is driven by clonal expansion of Escherichia coli ST38. In: 12th International Meeting on Microbial Epidemiological Markers; 2019 Sep 18-21; Dubrovnik, Croatia.
  3. Besser JM, Carleton HA, Trees E, Stroika SG, Hise K, Wise M, et al. Interpretation of Whole-Genome Sequencing for Enteric Disease Surveillance and Outbreak Investigation. Foodborne Pathog Dis. 2019;16(7):504-12.  https://doi.org/10.1089/fpd.2019.2650  PMID: 31246502 
  4. Gertler M, Czogiel I, Stark K, Wilking H. Assessment of recall error in self-reported food consumption histories among adults-Particularly delay of interviews decrease completeness of food histories-Germany, 2013. PLoS One. 2017;12(6):e0179121.  https://doi.org/10.1371/journal.pone.0179121  PMID: 28640839 
  5. Zhou Z, Alikhan NF, Mohamed K, Fan Y, Achtman M, Agama Study Group. The EnteroBase user’s guide, with case studies on Salmonella transmissions, Yersinia pestis phylogeny, and Escherichia core genomic diversity. Genome Res. 2020;30(1):138-52.  https://doi.org/10.1101/gr.251678.119  PMID: 31809257 
  6. Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, et al. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res. 2016;44(W1):W16-21.  https://doi.org/10.1093/nar/gkw387  PMID: 27141966 
  7. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, et al. The RAST Server: rapid annotations using subsystems technology. BMC Genomics. 2008;9(1):75.  https://doi.org/10.1186/1471-2164-9-75  PMID: 18261238 
  8. Masseron A, Poirel L, Falgenhauer L, Imirzalioglu C, Kessler J, Chakraborty T, et al. Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection. Diagn Microbiol Infect Dis. 2020;97(3):115059.  https://doi.org/10.1016/j.diagmicrobio.2020.115059  PMID: 32387849 
  9. Robert Koch Institut (RKI). Infektionsepidemiologisches Jahrbuch für 2018. [Infectious Disease Epidemiology Annual Report – 2018 ]. Berlin: RKI; 2019. Available from: https://www.rki.de/EN/Content/infections/epidemiology/inf_dis_Germany/yearbook/yearbook_summary_2018.html
  10. Abril D, Bustos Moya IG, Marquez-Ortiz RA, Josa Montero DF, Corredor Rozo ZL, Torres Molina I, et al. First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent. Antibiotics (Basel). 2019;8(4):222.  https://doi.org/10.3390/antibiotics8040222  PMID: 31766210 
  11. van Hattem JM, Arcilla MS, Bootsma MC, van Genderen PJ, Goorhuis A, Grobusch MP, et al. Prolonged carriage and potential onward transmission of carbapenemase-producing Enterobacteriaceae in Dutch travelers. Future Microbiol. 2016;11(7):857-64.  https://doi.org/10.2217/fmb.16.18  PMID: 27357522 
  12. Irrgang A, Tenhagen B-A, Pauly N, Schmoger S, Kaesbohrer A, Hammerl JA. Characterization of VIM-1-Producing E. coli Isolated From a German Fattening Pig Farm by an Improved Isolation Procedure. Front Microbiol. 2019;10(2256):2256.  https://doi.org/10.3389/fmicb.2019.02256  PMID: 31632372 
  13. Pulss S, Stolle I, Stamm I, Leidner U, Heydel C, Semmler T, et al. Multispecies and Clonal Dissemination of OXA-48 Carbapenemase in Enterobacteriaceae From Companion Animals in Germany, 2009-2016. Front Microbiol. 2018;9(1265):1265.  https://doi.org/10.3389/fmicb.2018.01265  PMID: 29963026 
  14. Berg ES, Wester AL, Ahrenfeldt J, Mo SS, Slettemeås JS, Steinbakk M, et al. Norwegian patients and retail chicken meat share cephalosporin-resistant Escherichia coli and IncK/blaCMY-2 resistance plasmids. Clin Microbiol Infect. 2017;23(6):407.e9-15.  https://doi.org/10.1016/j.cmi.2016.12.035  PMID: 28082191 
  15. Pietsch M, Irrgang A, Roschanski N, Brenner Michael G, Hamprecht A, Rieber H, et al. , RESET Study Group. Whole genome analyses of CMY-2-producing Escherichia coli isolates from humans, animals and food in Germany. BMC Genomics. 2018;19(1):601.  https://doi.org/10.1186/s12864-018-4976-3  PMID: 30092762 
  16. Hammerum AM, Porsbo LJ, Hansen F, Roer L, Kaya H, Henius A, et al. Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019. Euro Surveill. 2020;25(18):1900742.  https://doi.org/10.2807/1560-7917.ES.2020.25.18.1900742  PMID: 32400363 
  17. Masseron A, Poirel L, Falgenhauer L, Imirzalioglu C, Kessler J, Chakraborty T, et al. Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection. Diagn Microbiol Infect Dis. 2020;97(3):115059.  https://doi.org/10.1016/j.diagmicrobio.2020.115059  PMID: 32387849 
  18. Letunic I, Bork P. Interactive Tree Of Life (iTOL) v4: recent updates and new developments. Nucleic Acids Res. 2019;47(W1):W256-9.  https://doi.org/10.1093/nar/gkz239  PMID: 30931475 
/content/10.2807/1560-7917.ES.2020.25.25.2000923
Loading

Data & Media loading...

Supplementary data

Comment has been disabled for this content
Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error