1887
Rapid communication Open Access
Like 0

Abstract

The dissemination of carbapenem-producing Gram-negative bacteria is a major public health concern. We report the first detection of OXA-244-producing ST131 O16:H5 in three patients from two tertiary hospitals in the south-west of Germany. OXA-244 is emerging in Europe. Because of detection challenges, OXA-244-producing may be under-reported. The emergence of carbapenem resistance in a globally circulating high-risk clone, such as ST131 is of clinical relevance and should be monitored closely.

Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2020.25.46.2001815
2020-11-19
2020-11-27
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.46.2001815
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/25/46/eurosurv-25-46-5.html?itemId=/content/10.2807/1560-7917.ES.2020.25.46.2001815&mimeType=html&fmt=ahah

References

  1. Nicolas-Chanoine MH, Bertrand X, Madec JY. Escherichia coli ST131, an intriguing clonal group. Clin Microbiol Rev. 2014;27(3):543-74.  https://doi.org/10.1128/CMR.00125-13  PMID: 24982321 
  2. Peirano G, Bradford PA, Kazmierczak KM, Badal RE, Hackel M, Hoban DJ, et al. Global incidence of carbapenemase-producing Escherichia coli ST131. Emerg Infect Dis. 2014;20(11):1928-31.  https://doi.org/10.3201/eid2011.141388  PMID: 25340464 
  3. Poirel L, Potron A, Nordmann P. OXA-48-like carbapenemases: the phantom menace. J Antimicrob Chemother. 2012;67(7):1597-606.  https://doi.org/10.1093/jac/dks121  PMID: 22499996 
  4. Masseron A, Poirel L, Falgenhauer L, Imirzalioglu C, Kessler J, Chakraborty T, et al. Ongoing dissemination of OXA-244 carbapenemase-producing Escherichia coli in Switzerland and their detection. Diagn Microbiol Infect Dis. 2020;97(3):115059.  https://doi.org/10.1016/j.diagmicrobio.2020.115059  PMID: 32387849 
  5. Potron A, Poirel L, Dortet L, Nordmann P. Characterisation of OXA-244, a chromosomally-encoded OXA-48-like β-lactamase from Escherichia coli. Int J Antimicrob Agents. 2016;47(1):102-3.  https://doi.org/10.1016/j.ijantimicag.2015.10.015  PMID: 26655033 
  6. Kocer K, Boutin S, Dalpke AH, Heeg K, Mutters NT, Nurjadi D. Comparative genomic analysis reveals a high prevalence of inter-species in vivo transfer of carbapenem-resistance plasmids in patients with haematological malignancies. Clin Microbiol Infect. 2020;26(6):780.e1-8.  https://doi.org/10.1016/j.cmi.2019.10.014  PMID: 31654794 
  7. The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters, version 10.0, 2020. Available from: http://www.eucast.org/clinical_breakpoints/[REMOVED IF= FIELD]
  8. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, et al. SPAdes: a new genome assembly algorithm and its applications to single-cell sequencing. J Comput Biol. 2012;19(5):455-77.  https://doi.org/10.1089/cmb.2012.0021  PMID: 22506599 
  9. Page AJ, Cummins CA, Hunt M, Wong VK, Reuter S, Holden MT, et al. Roary: rapid large-scale prokaryote pan genome analysis. Bioinformatics. 2015;31(22):3691-3.  https://doi.org/10.1093/bioinformatics/btv421  PMID: 26198102 
  10. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068-9.  https://doi.org/10.1093/bioinformatics/btu153  PMID: 24642063 
  11. Feldgarden M, Brover V, Haft DH, Prasad AB, Slotta DJ, Tolstoy I, et al. Validating the AMRFinder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates. Antimicrob Agents Chemother. 2019;63(11):e00483-19.  https://doi.org/10.1128/AAC.00483-19  PMID: 31427293 
  12. Jia B, Raphenya AR, Alcock B, Waglechner N, Guo P, Tsang KK, et al. CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database. Nucleic Acids Res. 2017;45(D1):D566-73.  https://doi.org/10.1093/nar/gkw1004  PMID: 27789705 
  13. Gupta SK, Padmanabhan BR, Diene SM, Lopez-Rojas R, Kempf M, Landraud L, et al. ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes. Antimicrob Agents Chemother. 2014;58(1):212-20.  https://doi.org/10.1128/AAC.01310-13  PMID: 24145532 
  14. Zankari E, Hasman H, Cosentino S, Vestergaard M, Rasmussen S, Lund O, et al. Identification of acquired antimicrobial resistance genes. J Antimicrob Chemother. 2012;67(11):2640-4.  https://doi.org/10.1093/jac/dks261  PMID: 22782487 
  15. Hoyos-Mallecot Y, Naas T, Bonnin RA, Patino R, Glaser P, Fortineau N, et al. OXA-244-producing Escherichia coli isolates, a challenge for clinical microbiology laboratories. Antimicrob Agents Chemother. 2017;61(9):e00818-17.  https://doi.org/10.1128/AAC.00818-17  PMID: 28674064 
  16. Pierce VM, Simner PJ, Lonsway DR, Roe-Carpenter DE, Johnson JK, Brasso WB, et al. Modified carbapenem inactivation method for phenotypic detection of carbapenemase production among Enterobacteriaceae. J Clin Microbiol. 2017;55(8):2321-33.  https://doi.org/10.1128/JCM.00193-17  PMID: 28381609 
  17. Kremer K, Kramer R, Neumann B, Haller S, Pfennigwerth N, Werner G, et al. Rapid spread of OXA-244-producing Escherichia coli ST38 in Germany: insights from an integrated molecular surveillance approach; 2017 to January 2020. Euro Surveill. 2020;25(25):2000923.  https://doi.org/10.2807/1560-7917.ES.2020.25.25.2000923  PMID: 32613940 
  18. European Centre for Disease Prevention and Control (ECDC). Rapid risk assessment: Increase in OXA-244-producing Escherichia coli in the European Union/European Economic Area and the UK since 2013. Stockholm: ECDC; 18 Feb 2020. Available from: https://www.ecdc.europa.eu/en/publications-data/rapid-risk-assessment-increase-oxa-244-producing-escherichia-coli-eu-eea
  19. Fursova NK, Astashkin EI, Knyazeva AI, Kartsev NN, Leonova ES, Ershova ON, et al. The spread of bla OXA-48 and bla OXA-244 carbapenemase genes among Klebsiella pneumoniae, Proteus mirabilis and Enterobacter spp. isolated in Moscow, Russia. Ann Clin Microbiol Antimicrob. 2015;14(1):46.  https://doi.org/10.1186/s12941-015-0108-y  PMID: 26526183 
  20. Oteo J, Hernández JM, Espasa M, Fleites A, Sáez D, Bautista V, et al. Emergence of OXA-48-producing Klebsiella pneumoniae and the novel carbapenemases OXA-244 and OXA-245 in Spain. J Antimicrob Chemother. 2013;68(2):317-21.  https://doi.org/10.1093/jac/dks383  PMID: 23034714 
  21. Hammerum AM, Porsbo LJ, Hansen F, Roer L, Kaya H, Henius A, et al. Surveillance of OXA-244-producing Escherichia coli and epidemiologic investigation of cases, Denmark, January 2016 to August 2019. Euro Surveill. 2020;25(18).  https://doi.org/10.2807/1560-7917.ES.2020.25.18.1900742  PMID: 32400363 
  22. Falgenhauer L, Nordmann P, Imirzalioglu C, Yao Y, Falgenhauer J, Hauri AM, et al. Cross-border emergence of clonal lineages of ST38 Escherichia coli producing the OXA-48-like carbapenemase OXA-244 in Germany and Switzerland. Int J Antimicrob Agents. 2020;106157.  https://doi.org/10.1016/j.ijantimicag.2020.106157  PMID: 32919009 
  23. Emeraud C, Biez L, Girlich D, Jousset AB, Naas T, Bonnin RA, et al. Screening of OXA-244 producers, a difficult-to-detect and emerging OXA-48 variant? J Antimicrob Chemother. 2020;75(8):2120-3.  https://doi.org/10.1093/jac/dkaa155  PMID: 32363407 
  24. Pitout JD. Extraintestinal pathogenic Escherichia coli: an update on antimicrobial resistance, laboratory diagnosis and treatment. Expert Rev Anti Infect Ther. 2012;10(10):1165-76.  https://doi.org/10.1586/eri.12.110  PMID: 23199402 
  25. Albiger B, Glasner C, Struelens MJ, Grundmann H, Monnet DL, European Survey of Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) working group. Carbapenemase-producing Enterobacteriaceae in Europe: assessment by national experts from 38 countries, May 2015. Euro Surveill. 2015;20(45):30062.  https://doi.org/10.2807/1560-7917.ES.2015.20.45.30062  PMID: 26675038 
  26. Dahbi G, Mora A, Mamani R, López C, Alonso MP, Marzoa J, et al. Molecular epidemiology and virulence of Escherichia coli O16:H5-ST131: comparison with H30 and H30-Rx subclones of O25b:H4-ST131. Int J Med Microbiol. 2014;304(8):1247-57.  https://doi.org/10.1016/j.ijmm.2014.10.002  PMID: 25455219 
  27. Mora A, Dahbi G, López C, Mamani R, Marzoa J, Dion S, et al. Virulence patterns in a murine sepsis model of ST131 Escherichia coli clinical isolates belonging to serotypes O25b:H4 and O16:H5 are associated to specific virotypes. PLoS One. 2014;9(1):e87025.  https://doi.org/10.1371/journal.pone.0087025  PMID: 24498015 
  28. Matuschek E, Åhman J, Webster C, Kahlmeter G. Antimicrobial susceptibility testing of colistin - evaluation of seven commercial MIC products against standard broth microdilution for Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter spp. Clin Microbiol Infect. 2018;24(8):865-70. [REMOVED IF= FIELD][REMOVED IF= FIELD] https://doi.org/10.1016/j.cmi.2017.11.020  PMID: 29221995 
/content/10.2807/1560-7917.ES.2020.25.46.2001815
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error