1887
  1. Home
  2. Eurosurveillance
  3. Volume 26, Issue 36, 09/Sep/2021
  4. Article
Rapid communication Open Access
Like 0
Download

Abstract

We identified a novel gene cluster in a clinical isolate with vancomycin minimum inhibitory concentration (MIC) of 4 µg/mL. The ligase gene, , was part of a operon cluster of 4,589 bp on a putative novel integrative conjugative element located in a ca 98 kb genomic region presumed to be acquired by horizontal gene transfer from and sp. 499. Screening for genes in strains with borderline susceptibility to vancomycin is important.

© Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Loading

Article metrics loading...

/content/10.2807/1560-7917.ES.2021.26.36.2100767
2021-09-09
2024-04-26
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2021.26.36.2100767
Loading
Loading full text...

Full text loading...

/deliver/fulltext/eurosurveillance/26/36/eurosurv-26-36-2.html?itemId=/content/10.2807/1560-7917.ES.2021.26.36.2100767&mimeType=html&fmt=ahah

References

  1. Bonten MJ, Willems R, Weinstein RA. Vancomycin-resistant enterococci: why are they here, and where do they come from? Lancet Infect Dis. 2001;1(5):314-25.  https://doi.org/10.1016/S1473-3099(01)00145-1  PMID: 11871804 
  2. Zhou X, Willems RJL, Friedrich AW, Rossen JWA, Bathoorn E. Enterococcus faecium: from microbiological insights to practical recommendations for infection control and diagnostics. Antimicrob Resist Infect Control. 2020;9(1):130.  https://doi.org/10.1186/s13756-020-00770-1  PMID: 32778149 
  3. Tacconelli E, Carrara E, Savoldi A, Harbarth S, Mendelson M, Monnet DL, et al. Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis. Lancet Infect Dis. 2018;18(3):318-27.  https://doi.org/10.1016/S1473-3099(17)30753-3  PMID: 29276051 
  4. Xavier, B.B., et al., BacPipe: A rapid, user-friendly whole-genome sequencing pipeline for clinical diagnostic bacteriology. iScience. 2020;23(1):100769.
  5. Gibson MK, Forsberg KJ, Dantas G. Improved annotation of antibiotic resistance determinants reveals microbial resistomes cluster by ecology. ISME J. 2015;9(1):207-16.  https://doi.org/10.1038/ismej.2014.106  PMID: 25003965 
  6. Liu M, Li X, Xie Y, Bi D, Sun J, Li J, et al. ICEberg 2.0: an updated database of bacterial integrative and conjugative elements. Nucleic Acids Res. 2019;47(D1):D660-5.  https://doi.org/10.1093/nar/gky1123  PMID: 30407568 
  7. Arndt D, Grant JR, Marcu A, Sajed T, Pon A, Liang Y, et al. PHASTER: a better, faster version of the PHAST phage search tool. Nucleic Acids Res. 2016;44(W1):W16-21.  https://doi.org/10.1093/nar/gkw387  PMID: 27141966 
  8. Srinivasan V, Metcalf BJ, Knipe KM, Ouattara M, McGee L, Shewmaker PL, et al. vanG element insertions within a conserved chromosomal site conferring vancomycin resistance to Streptococcus agalactiae and Streptococcus anginosus. MBio. 2014;5(4):e01386-14.  https://doi.org/10.1128/mBio.01386-14  PMID: 25053786 
  9. Silva M, Machado MP, Silva DN, Rossi M, Moran-Gilad J, Santos S, et al. chewBBACA: A complete suite for gene-by-gene schema creation and strain identification. Microb Genom. 2018;4(3).  https://doi.org/10.1099/mgen.0.000166  PMID: 29543149 
  10. Zhou Z, Alikhan NF, Sergeant MJ, Luhmann N, Vaz C, Francisco AP, et al. GrapeTree: visualization of core genomic relationships among 100,000 bacterial pathogens. Genome Res. 2018;28(9):1395-404.  https://doi.org/10.1101/gr.232397.117  PMID: 30049790 
  11. Ahmed MO, Baptiste KE. Vancomycin-resistant enterococci: a review of antimicrobial resistance mechanisms and perspectives of human and animal health. Microb Drug Resist. 2018;24(5):590-606.  https://doi.org/10.1089/mdr.2017.0147  PMID: 29058560 
  12. Willems RJ, Top J, van Schaik W, Leavis H, Bonten M, Sirén J, et al. Restricted gene flow among hospital subpopulations of Enterococcus faecium. MBio. 2012;3(4):e00151-12.  https://doi.org/10.1128/mBio.00151-12  PMID: 22807567 
  13. Tamanai-Shacoori Z, Smida I, Bousarghin L, Loreal O, Meuric V, Fong SB, et al. Roseburia spp.: a marker of health? Future Microbiol. 2017;12(2):157-70.  https://doi.org/10.2217/fmb-2016-0130  PMID: 28139139 
  14. García-Solache M, Rice LB. The Enterococcus: a model of adaptability to its environment. Clin Microbiol Rev. 2019;32(2):e00058-18.  https://doi.org/10.1128/CMR.00058-18  PMID: 30700430 
  15. Gudeta DD, Moodley A, Bortolaia V, Guardabassi L. vanO, a new glycopeptide resistance operon in environmental Rhodococcus equi isolates. Antimicrob Agents Chemother. 2014;58(3):1768-70.  https://doi.org/10.1128/AAC.01880-13  PMID: 24342631 
  16. Sivertsen A, Pedersen T, Larssen KW, Bergh K, Rønning TG, Radtke A, et al. A Silenced vanA gene cluster on a transferable plasmid caused an outbreak of vancomycin-variable enterococci. Antimicrob Agents Chemother. 2016;60(7):4119-27.  https://doi.org/10.1128/AAC.00286-16  PMID: 27139479 
  17. Meziane-Cherif D, Saul FA, Haouz A, Courvalin P. Structural and functional characterization of VanG D-Ala:D-Ser ligase associated with vancomycin resistance in Enterococcus faecalis. J Biol Chem. 2012;287(45):37583-92.  https://doi.org/10.1074/jbc.M112.405522  PMID: 22969085 
  18. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-9.  https://doi.org/10.1093/molbev/msy096  PMID: 29722887 
Novel vancomycin resistance gene cluster in Enterococcus faecium ST1486, Belgium, June 2021
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Basil+Britto+Xavier&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Xavier Basil Britto</a>, <a href="/search?value1=Jasmine+Coppens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Coppens Jasmine</a>, <a href="/search?value1=Sien+De+Koster&option1=author&noRedirect=true" class="nonDisambigAuthorLink">De Koster Sien</a>, <a href="/search?value1=Sahaya+Glingston+Rajakani&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Rajakani Sahaya Glingston</a>, <a href="/search?value1=Sam+Van+Goethem&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Van Goethem Sam</a>, <a href="/search?value1=Samy+Mzougui&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mzougui Samy</a>, <a href="/search?value1=Ahalieyah+Anantharajah&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Anantharajah Ahalieyah</a>, <a href="/search?value1=Christine+Lammens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lammens Christine</a>, <a href="/search?value1=Katherine+Loens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Loens Katherine</a>, <a href="/search?value1=Youri+Glupczynski&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Glupczynski Youri</a>, <a href="/search?value1=Herman+Goossens&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Goossens Herman</a>, <a href="/search?value1=Veerle+Matheeussen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Matheeussen Veerle</a></span>. Novel vancomycin resistance gene cluster in Enterococcus faecium ST1486, Belgium, June 2021. <a href="/content/ecdc">Euro Surveill.</a> 2021;26(36):pii=2100767. <a href="https://doi.org/10.2807/1560-7917.ES.2021.26.36.2100767" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2021.26.36.2100767</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 29 Jul 2021 </span> </p>
/content/10.2807/1560-7917.ES.2021.26.36.2100767
/content/10.2807/1560-7917.ES.2021.26.36.2100767
Loading

Data & Media loading...

Submit comment
Close
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error