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Abstract

The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.

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/content/10.2807/1560-7917.ES.2022.27.35.2200636
2022-09-01
2025-05-21
/content/10.2807/1560-7917.ES.2022.27.35.2200636
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Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Nir+Paran&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Paran Nir</a>, <a href="/search?value1=Yfat+Yahalom-Ronen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Yahalom-Ronen Yfat</a>, <a href="/search?value1=Ohad+Shifman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Shifman Ohad</a>, <a href="/search?value1=Shirley+Lazar&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lazar Shirley</a>, <a href="/search?value1=Ronen+Ben-Ami&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ben-Ami Ronen</a>, <a href="/search?value1=Michal+Yakubovsky&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Yakubovsky Michal</a>, <a href="/search?value1=Itzchak+Levy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levy Itzchak</a>, <a href="/search?value1=Anat+Wieder-Feinsod&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wieder-Feinsod Anat</a>, <a href="/search?value1=Sharon+Amit&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Amit Sharon</a>, <a href="/search?value1=Michal+Katzir&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Katzir Michal</a>, <a href="/search?value1=Noga+Carmi-Oren&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Carmi-Oren Noga</a>, <a href="/search?value1=Ariela+Levcovich&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levcovich Ariela</a>, <a href="/search?value1=Mirit+Hershman-Sarafov&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hershman-Sarafov Mirit</a>, <a href="/search?value1=Alona+Paz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Paz Alona</a>, <a href="/search?value1=Rebecca+Thomas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Thomas Rebecca</a>, <a href="/search?value1=Hadas+Tamir&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tamir Hadas</a>, <a href="/search?value1=Lilach+Cherry-Mimran&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cherry-Mimran Lilach</a>, <a href="/search?value1=Noam+Erez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Erez Noam</a>, <a href="/search?value1=Sharon+Melamed&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Melamed Sharon</a>, <a href="/search?value1=Moria+Barlev-Gross&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barlev-Gross Moria</a>, <a href="/search?value1=Shay+Karmi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Karmi Shay</a>, <a href="/search?value1=Boaz+Politi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Politi Boaz</a>, <a href="/search?value1=Hagit+Achdout&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Achdout Hagit</a>, <a href="/search?value1=Shay+Weiss&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Weiss Shay</a>, <a href="/search?value1=Haim+Levy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levy Haim</a>, <a href="/search?value1=Ofir+Schuster&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schuster Ofir</a>, <a href="/search?value1=Adi+Beth-Din&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Beth-Din Adi</a>, <a href="/search?value1=Tomer+Israely&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Israely Tomer</a></span>. Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022. <a href="/content/ecdc">Euro Surveill.</a> 2022;27(35):pii=2200636. <a href="https://doi.org/10.2807/1560-7917.ES.2022.27.35.2200636" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2022.27.35.2200636</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 12 Aug 2022;&nbsp;&nbsp; <span class="generated">Accepted</span>: 01 Sept 2022 </span> </p>
/content/10.2807/1560-7917.ES.2022.27.35.2200636
/content/10.2807/1560-7917.ES.2022.27.35.2200636
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