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Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
- Nir Paran1,* , Yfat Yahalom-Ronen1,* , Ohad Shifman2 , Shirley Lazar2 , Ronen Ben-Ami3,10 , Michal Yakubovsky3 , Itzchak Levy4,10 , Anat Wieder-Feinsod4,10 , Sharon Amit5 , Michal Katzir6,10 , Noga Carmi-Oren7 , Ariela Levcovich7 , Mirit Hershman-Sarafov8,11 , Alona Paz8,11 , Rebecca Thomas9 , Hadas Tamir1 , Lilach Cherry-Mimran1 , Noam Erez1 , Sharon Melamed1 , Moria Barlev-Gross1 , Shay Karmi1 , Boaz Politi1 , Hagit Achdout1 , Shay Weiss1 , Haim Levy1 , Ofir Schuster1 , Adi Beth-Din2 , Tomer Israely1
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View Affiliations Hide AffiliationsAffiliations: 1 Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel 2 Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona, Israel 3 Infectious Diseases Department, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel 4 Infectious Disease Unit, Sheba Medical Center, Ramat Gan, Israel 5 Clinical Microbiology, Sheba Medical Center, Ramat Gan, Israel. 6 Infectious Disease unit, Meir Medical Center, Kfar Saba, Israel 7 Infectious Disease unit, Shamir (Assaf Harofeh) Medical Center, Be'er Ya'akov, Israel 8 Infectious Disease and Control Unit, Bnai Zion Medical Center, Haifa, Israel 9 Department of Emergency Medicine, Rabin Medical Center, Beilinson Hospital, Petah Tikva, Israel 10 Sackler medical school, Tel Aviv University, Tel Aviv, Israel 11 Rappaport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel* These authors contributed equally to this work and share first authorship.Correspondence:Adi Beth-Dinadib iibr.gov.il, Tomer Israelytomeri iibr.gov.il
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Citation style for this article: Paran Nir, Yahalom-Ronen Yfat, Shifman Ohad, Lazar Shirley, Ben-Ami Ronen, Yakubovsky Michal, Levy Itzchak, Wieder-Feinsod Anat, Amit Sharon, Katzir Michal, Carmi-Oren Noga, Levcovich Ariela, Hershman-Sarafov Mirit, Paz Alona, Thomas Rebecca, Tamir Hadas, Cherry-Mimran Lilach, Erez Noam, Melamed Sharon, Barlev-Gross Moria, Karmi Shay, Politi Boaz, Achdout Hagit, Weiss Shay, Levy Haim, Schuster Ofir, Beth-Din Adi, Israely Tomer. Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022. Euro Surveill. 2022;27(35):pii=2200636. https://doi.org/10.2807/1560-7917.ES.2022.27.35.2200636 Received: 12 Aug 2022; Accepted: 01 Sept 2022
Abstract
The current monkeypox virus global spread and lack of data regarding clinical specimens’ infectivity call for examining virus infectivity, and whether this correlates with results from PCR, the available diagnostic tool. We show strong correlation between viral DNA amount in clinical specimens and virus infectivity toward BSC-1 cell line. Moreover, we define a PCR threshold value (Cq ≥ 35, ≤ 4,300 DNA copies/mL), corresponding to negative viral cultures, which may assist risk-assessment and decision-making regarding protective-measures and guidelines for patients with monkeypox.
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/content/10.2807/1560-7917.ES.2022.27.35.2200636
2022-09-01
2023-09-26
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2022.27.35.2200636
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Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022
<p class="citation">
<span>Citation style for this article:</span>
<span class="meta-value authors">
<a href="/search?value1=Nir+Paran&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Paran Nir</a>,
<a href="/search?value1=Yfat+Yahalom-Ronen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Yahalom-Ronen Yfat</a>,
<a href="/search?value1=Ohad+Shifman&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Shifman Ohad</a>,
<a href="/search?value1=Shirley+Lazar&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Lazar Shirley</a>,
<a href="/search?value1=Ronen+Ben-Ami&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Ben-Ami Ronen</a>,
<a href="/search?value1=Michal+Yakubovsky&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Yakubovsky Michal</a>,
<a href="/search?value1=Itzchak+Levy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levy Itzchak</a>,
<a href="/search?value1=Anat+Wieder-Feinsod&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Wieder-Feinsod Anat</a>,
<a href="/search?value1=Sharon+Amit&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Amit Sharon</a>,
<a href="/search?value1=Michal+Katzir&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Katzir Michal</a>,
<a href="/search?value1=Noga+Carmi-Oren&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Carmi-Oren Noga</a>,
<a href="/search?value1=Ariela+Levcovich&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levcovich Ariela</a>,
<a href="/search?value1=Mirit+Hershman-Sarafov&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Hershman-Sarafov Mirit</a>,
<a href="/search?value1=Alona+Paz&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Paz Alona</a>,
<a href="/search?value1=Rebecca+Thomas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Thomas Rebecca</a>,
<a href="/search?value1=Hadas+Tamir&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Tamir Hadas</a>,
<a href="/search?value1=Lilach+Cherry-Mimran&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cherry-Mimran Lilach</a>,
<a href="/search?value1=Noam+Erez&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Erez Noam</a>,
<a href="/search?value1=Sharon+Melamed&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Melamed Sharon</a>,
<a href="/search?value1=Moria+Barlev-Gross&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barlev-Gross Moria</a>,
<a href="/search?value1=Shay+Karmi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Karmi Shay</a>,
<a href="/search?value1=Boaz+Politi&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Politi Boaz</a>,
<a href="/search?value1=Hagit+Achdout&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Achdout Hagit</a>,
<a href="/search?value1=Shay+Weiss&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Weiss Shay</a>,
<a href="/search?value1=Haim+Levy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Levy Haim</a>,
<a href="/search?value1=Ofir+Schuster&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Schuster Ofir</a>,
<a href="/search?value1=Adi+Beth-Din&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Beth-Din Adi</a>,
<a href="/search?value1=Tomer+Israely&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Israely Tomer</a></span>.
Monkeypox DNA levels correlate with virus infectivity in clinical samples, Israel, 2022.
<a href="/content/ecdc">Euro Surveill.</a> 2022;27(35):pii=2200636.
<a href="https://doi.org/10.2807/1560-7917.ES.2022.27.35.2200636" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2022.27.35.2200636</a>
<span class="ES_Article_citation">
<span class="generated">Received</span>: 12 Aug 2022;
<span class="generated">Accepted</span>: 01 Sept 2022
</span>
</p>
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