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Abstract

To advance our understanding of respiratory syncytial virus (RSV) impact through genomic surveillance, we describe two PCR-based sequencing systems, (i) RSVAB-WGS for generic whole-genome sequencing and (ii) RSVAB-GF, which targets major viral antigens, G and F, and is used as a complement for challenging cases with low viral load. These methods monitor RSV genetic diversity to inform molecular epidemiology, vaccine effectiveness and treatment strategies, contributing also to the standardisation of surveillance in a new era of vaccines.

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/content/10.2807/1560-7917.ES.2023.28.49.2300637
2023-12-07
2024-05-02
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2023.28.49.2300637
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Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Mar%C3%ADa+Iglesias-Caballero&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Iglesias-Caballero María</a>, <a href="/search?value1=Sara+Camarero-Serrano&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Camarero-Serrano Sara</a>, <a href="/search?value1=Sarai+Varona&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Varona Sarai</a>, <a href="/search?value1=Vicente+Mas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Mas Vicente</a>, <a href="/search?value1=Cristina+Calvo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Calvo Cristina</a>, <a href="/search?value1=Mar%C3%ADa+Luz+Garc%C3%ADa&option1=author&noRedirect=true" class="nonDisambigAuthorLink">García María Luz</a>, <a href="/search?value1=Juan+Garc%C3%ADa-Costa&option1=author&noRedirect=true" class="nonDisambigAuthorLink">García-Costa Juan</a>, <a href="/search?value1=Sonia+V%C3%A1zquez-Mor%C3%B3n&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Vázquez-Morón Sonia</a>, <a href="/search?value1=Sara+Monz%C3%B3n&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Monzón Sara</a>, <a href="/search?value1=Albert+Campoy&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Campoy Albert</a>, <a href="/search?value1=Isabel+Cuesta&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Cuesta Isabel</a>, <a href="/search?value1=Francisco+Pozo&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Pozo Francisco</a>, <a href="/search?value1=Inmaculada+Casas&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Casas Inmaculada</a></span>. Genomic characterisation of respiratory syncytial virus: a novel system for whole genome sequencing and full-length G and F gene sequences. <a href="/content/ecdc">Euro Surveill.</a> 2023;28(49):pii=2300637. <a href="https://doi.org/10.2807/1560-7917.ES.2023.28.49.2300637" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2023.28.49.2300637</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 16 Nov 2023;&nbsp;&nbsp; <span class="generated">Accepted</span>: 30 Nov 2023 </span> </p>
/content/10.2807/1560-7917.ES.2023.28.49.2300637
/content/10.2807/1560-7917.ES.2023.28.49.2300637
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