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Abstract

Real-time PCR is one of the most widely used techniques to diagnose measles cases. Here we report measles virus variants with three genetic mutations in the reverse primer annealing site of a widely used PCR. The mutations result in a slight loss of the PCR sensitivity. Variants bearing the three mutations presently circulate in different countries since at least the end of 2021. Our findings highlight the usefulness of molecular surveillance in monitoring if oligonucleotides in diagnostic tests remain adequate.

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/content/10.2807/1560-7917.ES.2024.29.5.2400034
2024-02-01
2024-02-22
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2024.29.5.2400034
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References

  1. World Health Organization (WHO). Manual for the laboratory diagnosis of measles and rubella virus infection, 3rd edition. Geneva: WHO; 2018. Available at: https://www.technet-21.org/en/manual-introduction
  2. Hummel KB, Lowe L, Bellini WJ, Rota PA. Development of quantitative gene-specific real-time RT-PCR assays for the detection of measles virus in clinical specimens. J Virol Methods. 2006;132(1-2):166-73.  https://doi.org/10.1016/j.jviromet.2005.10.006  PMID: 16274752 
  3. World Health Organization (WHO). Update of the nomenclature for describing the genetic characteristics of wild-type measles viruses: new genotypes and reference strains. Wkly Epidemiol Rec. 2003;78(27):229-32. PMID: 12866261 
  4. Benson DA, Cavanaugh M, Clark K, Karsch-Mizrachi I, Lipman DJ, Ostell J, et al. GenBank. Nucleic Acids Res. 2013;41(Database issue) D1;D36-42. PMID: 23193287 
  5. Genetic diversity of wild-type measles viruses and the global measles nucleotide surveillance database (MeaNS). Wkly Epidemiol Rec. 2015;90(30):373-80. PMID: 26211016 
  6. Richard JL, Mäusezahl M, Basler S, Eckert N. Approaching measles elimination in Switzerland: changing epidemiology 2007-2018. Swiss Med Wkly. 2019;149(2324):w20102-20102.  https://doi.org/10.4414/smw.2019.20102  PMID: 31185128 
  7. World Health Organization (WHO). Update: circulation of active genotypes of measles virus and recommendations for use of sequence analysis to monitor viral transmission. Wkly Epidemiol Rec.2022;97(39):485-92.
  8. El Mubarak HS, De Swart RL, Osterhaus ADME, Schutten M. Development of a semi-quantitative real-time RT-PCR for the detection of measles virus. J Clin Virol. 2005;32(4):313-7.  https://doi.org/10.1016/j.jcv.2004.08.017  PMID: 15780811 
  9. Tahan S, Parikh BA, Droit L, Wallace MA, Burnham CD, Wang D. SARS-CoV-2 E Gene Variant Alters Analytical Sensitivity Characteristics of Viral Detection Using a Commercial Reverse Transcription-PCR Assay. J Clin Microbiol. 2021;59(7):e0007521.  https://doi.org/10.1128/JCM.00075-21  PMID: 33903167 
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