Genesis of the novel human-infecting influenza A(H10N8) virus and potential genetic diversity of the virus in poultry, China

W Qi; X Zhou; W Shi; L Huang; W Xia; D Liu; H Li; S Chen; F Lei; L Cao; J Wu; F He; W Song; Q Li; H Li; M Liao; M Liu

doi:10.2807/1560-7917.ES2014.19.25.20841

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Genesis of the novel human-infecting influenza A(H10N8) virus and potential genetic diversity of the virus in poultry, China

W Qi^1,2, X Zhou^2,3, W Shi^2,4, L Huang¹, W Xia³, D Liu⁵, H Li¹, S Chen³, F Lei⁶, L Cao⁷, J Wu³, F He³, W Song³, Q Li¹, H Li³, M Liao^1,2, M Liu^2,3
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Affiliations: ¹ National and Local Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China ² These authors contributed equally to this article ³ Nanchang Center for Disease Control and Prevention, Nanchang, China ⁴ School of Basic Medical Sciences, Taishan Medical College, Taian, Shandong, China ⁵ Network Information Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China ⁶ CAS Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China ⁷ Guangzhou Center for Disease Control and Prevention, Guangzhou, China

Correspondence:
M Liu
mingbinliu 126.com
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Citation style for this article: Qi W, Zhou X, Shi W, Huang L, Xia W, Liu D, Li H, Chen S, Lei F, Cao L, Wu J, He F, Song W, Li Q, Li H, Liao M, Liu M. Genesis of the novel human-infecting influenza A(H10N8) virus and potential genetic diversity of the virus in poultry, China. Euro Surveill. 2014;19(25):pii=20841. https://doi.org/10.2807/1560-7917.ES2014.19.25.20841 Received: 07 Mar 2014

Abstract

Human infection with a novel influenza A(H10N8) virus was first described in China in December 2013. However, the origin and genetic diversity of this virus is still poorly understood. We performed a phylogenetic analysis and coalescent analysis of two viruses from the first case of influenza A(H10N8) (A/Jiangxi-Donghu/346-1/2013 and A/Jiangxi-Donghu/346-2/2013 and a novel A(H10N8) virus (A/chicken/Jiangxi/102/2013) isolated from a live poultry market that the patient had visited. The haemagglutinin (HA), neuraminidase (NA), PA subunit of the virus polymerase complex, nucleoprotein (NP), M and nonstructural protein (NS) genes of the three virus strains shared the same genetic origins. The origins of their HA and NA genes were similar: originally from wild birds to ducks, and then to chickens. The PA, NP, M, and NS genes were similar to those of chicken influenza A(H9N2) viruses. Coalescent analyses showed that the reassortment of these genes from A(H9N2) to A(H10N8) might have occurred at least twice. However, the PB1 and PB2 genes of the chicken A(H10N8) virus most likely originated from H7-like viruses of ducks, while those of the viruses from the case most likely stemmed from A(H9N2) viruses circulating in chickens. The oseltamivir-resistance mutation, R292K (R291K in A(H10N8) numbering) in the NA protein, occurred after four days of oseltamivir treatment. It seems that A(H10N8) viruses might have become established among poultry and their genetic diversity might be much higher than what we have observed.