False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011

D M Whiley; A Limnios; N J Moon; N Gehrig; N Goire; T Hogan; A Lam; K Jacob; S B Lambert; M D Nissen; T P Sloots

doi:10.2807/ese.16.21.19874-en

Rapid communications Open Access

Like 0

Download

False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011
D M Whiley^1,2,3 , A Limnios⁴ , N J Moon⁵ , N Gehrig⁵ , N Goire^1,2,3 , T Hogan⁴ , A Lam⁴ , K Jacob^1,2,3 , S B Lambert^1,2,3 , M D Nissen^1,2,3,5 , T P Sloots^1,2,3,5
View Affiliations Hide Affiliations

Affiliations: ¹ Queensland Paediatric Infectious Diseases Laboratory, Queensland Children’s Health Services, Queensland, Australia ² Queensland Children’s Medical Research Institute, The University of Queensland, Queensland, Australia ³ Clinical Medical Virology Centre, Sir Albert Sakzewski Virus Research Centre, The University of Queensland, Queensland, Australia ⁴ World Health Organization Collaborating Centre for Sexually Transmitted Diseases, Sydney, Microbiology Department, South Eastern Area Laboratory Services, The Prince of Wales Hospital, Sydney, New South Wales, Australia ⁵ Microbiology Department, Hunter Area Pathology Service, Division of Pathology North, Newcastle, New South Wales, Australia

Correspondence:
D Whiley
d.whiley uq.edu.au
View Citation Hide Citation

Citation style for this article: Whiley D M, Limnios A, Moon N J, Gehrig N, Goire N, Hogan T, Lam A, Jacob K, Lambert S B, Nissen M D, Sloots T P. False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011. Euro Surveill. 2011;16(21):pii=19874. https://doi.org/10.2807/ese.16.21.19874-en Received: 19 May 2011

Abstract

The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. gonorrhoeae porA pseudogene PCR false-negative result caused by sequence variation, and in the same organism, the first description of a clinical N. gonorrhoeae strain harbouring an N. meningitidis porA sequence.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Article metrics loading...

/content/10.2807/ese.16.21.19874-en

2011-05-26

2021-07-31

Click here for cited by

http://instance.metastore.ingenta.com/content/10.2807/ese.16.21.19874-en

From This Site

/content/10.2807/ese.16.21.19874-en

dcterms_title,dcterms_subject,pub_keyword

-contentType:Journal -contentType:Contributor -contentType:Concept -contentType:Institution

10

5
PubMed
Google Scholar

Full text loading...

/deliver/fulltext/eurosurveillance/16/21/art19874-en.htm?itemId=/content/10.2807/ese.16.21.19874-en&mimeType=html&fmt=ahah

Submit comment

Comment moderation successfully completed

False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011

Abstract

Click here for cited by

From This Site

PubMed

Google Scholar