False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011

D M Whiley; A Limnios; N J Moon; N Gehrig; N Goire; T Hogan; A Lam; K Jacob; S B Lambert; M D Nissen; T P Sloots

doi:10.2807/ese.16.21.19874-en

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False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011

D M Whiley^1,2,3, A Limnios⁴, N J Moon⁵, N Gehrig⁵, N Goire^1,2,3, T Hogan⁴, A Lam⁴, K Jacob^1,2,3, S B Lambert^1,2,3, M D Nissen^1,2,3,5, T P Sloots^1,2,3,5
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Affiliations: ¹ Queensland Paediatric Infectious Diseases Laboratory, Queensland Children’s Health Services, Queensland, Australia ² Queensland Children’s Medical Research Institute, The University of Queensland, Queensland, Australia ³ Clinical Medical Virology Centre, Sir Albert Sakzewski Virus Research Centre, The University of Queensland, Queensland, Australia ⁴ World Health Organization Collaborating Centre for Sexually Transmitted Diseases, Sydney, Microbiology Department, South Eastern Area Laboratory Services, The Prince of Wales Hospital, Sydney, New South Wales, Australia ⁵ Microbiology Department, Hunter Area Pathology Service, Division of Pathology North, Newcastle, New South Wales, Australia

Correspondence:
D Whiley
d.whiley uq.edu.au
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Citation style for this article: Whiley D M, Limnios A, Moon N J, Gehrig N, Goire N, Hogan T, Lam A, Jacob K, Lambert S B, Nissen M D, Sloots T P. False-negative results using Neisseria gonorrhoeae porA pseudogene PCR - a clinical gonococcal isolate with an N. meningitidis porA sequence, Australia, March 2011. Euro Surveill. 2011;16(21):pii=19874. https://doi.org/10.2807/ese.16.21.19874-en Received: 19 May 2011; Accepted:

Abstract

The gonococcal porA pseudogene is a popular target for in-house Neisseria gonorrhoeae PCR methods. With this study we present two novel findings: the first case of an N. gonorrhoeae porA pseudogene PCR false-negative result caused by sequence variation, and in the same organism, the first description of a clinical N. gonorrhoeae strain harbouring an N. meningitidis porA sequence.