Rapid communication Open Access
Like 0


The current Zika virus (ZIKV) epidemic in the Americas caused an increase in diagnostic requests in European countries. Here we demonstrate high specificity of the Euroimmun anti-ZIKV IgG and IgM ELISA tests using putative cross-reacting sera of European patients with antibodies against tick-borne encephalitis virus, dengue virus, yellow fever virus and hepatitis C virus. This test may aid in counselling European travellers returning from regions where ZIKV is endemic.


Article metrics loading...

Loading full text...

Full text loading...



  1. European Centre for Disease Prevention and Control (ECDC). Zika virus infection: Factsheet for health professionals. Stockholm: ECDC. [Accessed: 28 Mar 2016]. Available from: http://ecdc.europa.eu/en/healthtopics/zika_virus_infection/factsheet-health-professionals/Pages/factsheet_health_professionals.aspx#sthash.Ovu33Mb6.dpuf
  2. Vorou R. Letter to the editor: diagnostic challenges to be considered regarding Zika virus in the context of the presence of the vector Aedes albopictus in Europe. Euro Surveill. 2016;21(10):30161. http://dx.doi.org/ https://doi.org/10.2807/1560-7917.ES.2016.21.10.30161 
  3. Serological diagnosis of Zika virus infections. Press release. Luebeck: Euroimmun; 2016. Available from: https://www.euroimmun.com/index.php?eID=dumpFile&t=f&f=3013&token=6cc0bb2eb7c10f484ae50ad91e69cd6424088529
  4. Tappe D, Rissland J, Gabriel M, Emmerich P, Gunther S, Held G, et al. First case of laboratory-confirmed Zika virus infection imported into Europe, November 2013. Euro Surveill. 2014;19(4):20685.  https://doi.org/10.2807/1560-7917.ES2014.19.4.20685  PMID: 24507467 
  5. Brasil P, Pereira JP Jr, Raja Gabaglia C, Damasceno L, Wakimoto M, Ribeiro Nogueira RM, et al. Zika Virus Infection in Pregnant Women in Rio de Janeiro - Preliminary Report. N Engl J Med. 2016;NEJMoa1602412.  https://doi.org/10.1056/NEJMoa1602412  PMID: 26943629 
  6. Rasmussen SA, Jamieson DJ, Honein MA, Petersen LR. Zika Virus and Birth Defects - Reviewing the Evidence for Causality. N Engl J Med. 2016;NEJMsr1604338.  https://doi.org/10.1056/NEJMsr1604338  PMID: 27074377 
  7. Zika virus infection – short facts assembled by the Society for Virology in Germany. Erlangen: European Society for Virology [Accessed: 19 Apr 2016]. Available from: http://www.eusv.eu/zika-virus-infection-short-facts-assembled-by-the-society-for-virology-in-germany/
  8. Allwinn R, Doerr HW, Emmerich P, Schmitz H, Preiser W. Cross-reactivity in flavivirus serology: new implications of an old finding? Med Microbiol Immunol (Berl). 2002;190(4):199-202.  https://doi.org/10.1007/s00430-001-0107-9  PMID: 12005333 
  9. Koraka P, Zeller H, Niedrig M, Osterhaus AD, Groen J. Reactivity of serum samples from patients with a flavivirus infection measured by immunofluorescence assay and ELISA. Microbes Infect. 2002;4(12):1209-15.  https://doi.org/10.1016/S1286-4579(02)01647-7  PMID: 12467761 
  10. Heinz FX, Stiasny K, Holzmann H, Grgic-Vitek M, Kriz B, Essl A, et al. Vaccination and tick-borne encephalitis, central Europe. Emerg Infect Dis. 2013;19(1):69-76.  https://doi.org/10.3201/eid1901.120458  PMID: 23259984 
  11. Venturi G, Zammarchi L, Fortuna C, Remoli ME, Benedetti E, Fiorentini C, et al. Authors’ reply: diagnostic challenges to be considered regarding Zika virus in the context of the presence of the vector Aedes albopictus in Europe. Euro Surveill. 2016;21(10):30163.  https://doi.org/10.2807/1560-7917.ES.2016.21.10.30163  PMID: 26988337 
  12. Musso D, Roche C, Nhan TX, Robin E, Teissier A, Cao-Lormeau VM. Detection of Zika virus in saliva. J Clin Virol. 2015;68:53-5.  https://doi.org/10.1016/j.jcv.2015.04.021  PMID: 26071336 
  13. Alidjinou EK, Lazrek M, Libier L, Bocket L, Dewilde A, Engelmann I, et al. A patient with fever, abdominal pain and bicytopenia: Trouble once again with these IgM antibodies! J Clin Virol. 2016;75:60-3.  https://doi.org/10.1016/j.jcv.2015.11.025  PMID: 26679224 
  14. Reusken C, Mou H, Godeke GJ, van der Hoek L, Meyer B, Müller MA, et al. Specific serology for emerging human coronaviruses by protein microarray. Euro Surveill. 2013;18(14):20441.  https://doi.org/10.2807/1560-7917.ES2013.18.14.20441  PMID: 23594517 

Data & Media loading...

Submit comment
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error