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Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
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View Affiliations Hide AffiliationsJesper LarsenJRL ssi.dk
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Citation style for this article: . Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016. Euro Surveill. 2017;22(28):pii=30573. https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573 Received: 13 Jun 2017; Accepted: 11 Jul 2017
Abstract
We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

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