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Abstract

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

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/content/10.2807/1560-7917.ES.2017.22.28.30573
2017-07-13
2025-05-16
/content/10.2807/1560-7917.ES.2017.22.28.30573
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Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016
<p class="citation"> <span>Citation style for this article:</span> <span class="meta-value authors"> <a href="/search?value1=Jesper+Larsen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larsen Jesper</a>, <a href="/search?value1=Marianne+Sunde&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Sunde Marianne</a>, <a href="/search?value1=Md+Zohorul+Islam&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Islam Md Zohorul</a>, <a href="/search?value1=Anne+Margrete+Urdahl&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Urdahl Anne Margrete</a>, <a href="/search?value1=Aina+Steihaug+Barstad&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Barstad Aina Steihaug</a>, <a href="/search?value1=Anders+Rhod+Larsen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Larsen Anders Rhod</a>, <a href="/search?value1=Carl+Andreas+Gr%C3%B8ntvedt&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Grøntvedt Carl Andreas</a>, <a href="/search?value1=%C3%98ystein+Angen&option1=author&noRedirect=true" class="nonDisambigAuthorLink">Angen Øystein</a></span>. Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016. <a href="/content/ecdc">Euro Surveill.</a> 2017;22(28):pii=30573. <a href="https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573" title="doi link" target="_blank">https://doi.org/10.2807/1560-7917.ES.2017.22.28.30573</a> <span class="ES_Article_citation"> <span class="generated">Received</span>: 13 Jun 2017;&nbsp;&nbsp; <span class="generated">Accepted</span>: 11 Jul 2017 </span> </p>
/content/10.2807/1560-7917.ES.2017.22.28.30573
/content/10.2807/1560-7917.ES.2017.22.28.30573
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