Rapid communication Open Access
Like 0


During summer 2019, three patients residing by Tisvilde Hegn, Denmark were hospitalised with tick-borne encephalitis (TBE) after tick bites. A new TBE virus (TBEV) micro-focus was identified in tick nymphs collected around a playground in Tisvilde Hegn forest. Estimated TBEV prevalence was 8%, higher than in endemic areas around Europe. Whole genome sequencing showed clustering to a TBEV strain from Norway. This is the second time TBEV is found in outside Bornholm, Denmark.


Article metrics loading...

Loading full text...

Full text loading...



  1. Laursen K, Knudsen JD. Tick-borne encephalitis: a retrospective study of clinical cases in Bornholm, Denmark. Scand J Infect Dis. 2003;35(5):354-7.  https://doi.org/10.1080/00365540310005305  PMID: 12875531 
  2. Andersen NS, Larsen SL, Olesen CR, Stiasny K, Kolmos HJ, Jensen PM, et al. Continued expansion of tick-borne pathogens: Tick-borne encephalitis virus complex and Anaplasma phagocytophilum in Denmark. Ticks Tick Borne Dis. 2019;10(1):115-23.  https://doi.org/10.1016/j.ttbdis.2018.09.007  PMID: 30245088 
  3. Niedrig M, Avsic T, Aberle SW, Ferenczi E, Labuda M, Rozentale B, et al. Quality control assessment for the serological diagnosis of tick borne encephalitis virus infections. J Clin Virol. 2007;38(3):260-4.  https://doi.org/10.1016/j.jcv.2006.12.013  PMID: 17267281 
  4. Fomsgaard A, Christiansen C, Bødker R. First identification of tick-borne encephalitis in Denmark outside of Bornholm, August 2009. Euro Surveill. 2009;14(36):19325. PMID: 19758543 
  5. Fomsgaard A, Fertner ME, Essbauer S, Nielsen AY, Frey S, Lindblom P, et al. Tick-borne encephalitis virus, Zealand, Denmark, 2011. Emerg Infect Dis. 2013;19(7):1171-3.  https://doi.org/10.3201/eid1907.130092  PMID: 23764123 
  6. Sergeant ESG. 2019. Epitools epidemiological calculators. Bruce ACT Australia: Ausvet Pty Ltd. [Accessed 13 Oct 2019]. Available from: http://epitools.ausvet.com.au
  7. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol. 2018;35(6):1547-9.  https://doi.org/10.1093/molbev/msy096  PMID: 29722887 
  8. European Centre for Disease Prevention and Control (ECDC). 2018. Tick-borne encephalitis. Annual Epidemiological report for 2017. Stockholm: ECDC; 2019. [Accessed 15 Oct 2019]. Available from: https://www.ecdc.europa.eu/sites/default/files/documents/AER_for_2017-tick-borne-encephalitis_0.pdf
  9. Lindquist L, Vapalahti O. Tick-borne encephalitis. Lancet. 2008;371(9627):1861-71.  https://doi.org/10.1016/S0140-6736(08)60800-4  PMID: 18514730 
  10. Jaenson TGT, Hjertqvist M, Bergström T, Lundkvist A. Why is tick-borne encephalitis increasing? A review of the key factors causing the increasing incidence of human TBE in Sweden. Parasit Vectors. 2012;5(1):184.  https://doi.org/10.1186/1756-3305-5-184  PMID: 22937961 
  11. Sidorenko M, Radzievskaja J, Rosef O, Paulauskas A. Investigation of the tick-borne encephalitis virus in Norway. Biologija (Vilnius). 2018;64(2):172-8.  https://doi.org/10.6001/biologija.v64i2.3741 
  12. Kjær LJ, Soleng A, Edgar KS, Lindstedt HEH, Paulsen KM, Andreassen ÅK, et al. A large-scale screening for the taiga tick, Ixodes persulcatus, and the meadow tick, Dermacentor reticulatus, in southern Scandinavia, 2016. Parasit Vectors. 2019;12(1):338.  https://doi.org/10.1186/s13071-019-3596-3  PMID: 31288866 
  13. Petersen A, Rosenstierne MW, Rasmussen M, Fuursted K, Nielsen HV, O’Brien Andersen L, et al. Field samplings of Ixodes ricinus ticks from a tick-borne encephalitis virus micro-focus in Northern Zealand, Denmark. Ticks Tick Borne Dis. 2019;10(5):1028-32.  https://doi.org/10.1016/j.ttbdis.2019.05.005  PMID: 31151922 
  14. Laugesen NG, Stenør C. [Tick-borne encephalitis-associated meningoradiculoneuritis acquired in the south-western part of Denmark]. Ugeskr Laeger. 2019;181(27):V03190197. PMID: 31267941 
  15. Andersen NS, Bestehorn M, Chitimia-Dobler L, Kolmos HJ, Jensen PM, Dobler G, et al. Phylogenetic characterization of tick-borne encephalitis virus from Bornholm, Denmark. Ticks Tick Borne Dis. 2019;10(3):533-9.  https://doi.org/10.1016/j.ttbdis.2018.12.008  PMID: 30704909 

Data & Media loading...

Submit comment
Comment moderation successfully completed
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error