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Abstract

Facing the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), high-volume respiratory testing is demanded in laboratories worldwide. We evaluated the performance of a molecular assay for the detection of SARS-CoV-2 on a high-throughput platform, the cobas 6800, using the ‘open channel’ for integration of a laboratory-developed assay. We observed good analytical performance in clinical specimens. The fully automated workflow enables high-throughput testing with minimal hands-on time, while offering fast and reliable results.

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/content/10.2807/1560-7917.ES.2020.25.9.2000152
2020-03-05
2020-11-29
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.9.2000152
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References

  1. Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al. China Novel Coronavirus Investigating and Research Team. A novel coronavirus from patients with pneumonia in China, 2019. N Engl J Med. 2020;382(8):727-33.  https://doi.org/10.1056/NEJMoa2001017  PMID: 31978945 
  2. Gorbalenya AE, Baker SC, Baric RS, de Groot RJ, Drosten C, Gulyaeva AA, et al. Severe acute respiratory syndrome-related coronavirus: The species and its viruses – a statement of the Coronavirus Study Group. bioRxiv. 2020;937862: (Preprint).  https://doi.org/10.1101/2020.02.07.937862 
  3. Mahase E. China coronavirus: WHO declares international emergency as death toll exceeds 200. BMJ. 2020;368:m408.  https://doi.org/10.1136/bmj.m408  PMID: 32005727 
  4. Corman VM, Landt O, Kaiser M, Molenkamp R, Meijer A, Chu DKW, et al. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR. Euro Surveill. 2020;25(3):2000045.  https://doi.org/10.2807/1560-7917.ES.2020.25.3.2000045  PMID: 31992387 
  5. Centers for Disease Control and Prevention (CDC). Research use only 2019-novel coronavirus (2019-nCoV) real-time rt-pcr primer and probe information. Atlanta: CDC; 2020.Available from: https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html
  6. Boujnan M, Duits AJ, Koppelman MHGM. Zika virus RNA polymerase chain reaction on the utility channel of a commercial nucleic acid testing system. Transfusion. 2018;58(3):641-8.  https://doi.org/10.1111/trf.14460  PMID: 29322525 
  7. Romette JL, Prat CM, Gould EA, de Lamballerie X, Charrel R, Coutard B, et al. The European Virus Archive goes global: A growing resource for research. Antiviral Res. 2018;158:127-34.  https://doi.org/10.1016/j.antiviral.2018.07.017  PMID: 30059721 
  8. Enserink M. War stories. Science. 2013;339(6125):1264-8.  https://doi.org/10.1126/science.339.6125.1264  PMID: 23493690 
  9. ProMED-mail. Novel coronavirus (37): China, global, cruise ship, clinical presentation, WHO. Archive Number: 20200208.6967526. 8 Feb 2020. Available from: https://promedmail.org/promed-post/?id=6967526
  10. Cobb B, Simon CO, Stramer SL, Body B, Mitchell PS, Reisch N, et al. The cobas® 6800/8800 System: a new era of automation in molecular diagnostics. Expert Rev Mol Diagn. 2017;17(2):167-80.  https://doi.org/10.1080/14737159.2017.1275962  PMID: 28043179 
  11. Marlowe EM, Hardy D, Krevolin M, Gohl P, Bertram A, Arcenas R, et al. High-throughput testing of urogenital and extragenital specimens for detection of Chlamydia trachomatis and Neisseria gonorrhoeae with cobas® CT/NG. Eur J Microbiol Immunol (Bp). 2017;7(3):176-86.  https://doi.org/10.1556/1886.2017.00018  PMID: 29034107 
  12. Greub G, Sahli R, Brouillet R, Jaton K. Ten years of R&D and full automation in molecular diagnosis. Future Microbiol. 2016;11(3):403-25.  https://doi.org/10.2217/fmb.15.152  PMID: 27028061 
  13. Eigner U, Reucher S, Hefner N, Staffa-Peichl S, Kolb M, Betz U, et al. Clinical evaluation of multiplex RT-PCR assays for the detection of influenza A/B and respiratory syncytial virus using a high throughput system. J Virol Methods. 2019;269:49-54.  https://doi.org/10.1016/j.jviromet.2019.03.015  PMID: 30946852 
  14. Yao JD, Young S, Heilek GM, Marino E, Paxinos EE, Marins EG, et al. Diagnosis and monitoring of HCV infection using the cobas® HCV test for use on the cobas® 6800/8800 systems. J Clin Virol. 2018;102:63-9.  https://doi.org/10.1016/j.jcv.2018.02.017  PMID: 29518694 
  15. Vermehren J, Stelzl E, Maasoumy B, Michel-Treil V, Berkowski C, Marins EG, et al. Multicenter comparison study of both analytical and clinical performance across four Roche hepatitis C virus RNA assays utilizing different platforms. J Clin Microbiol. 2017;55(4):1131-9.  https://doi.org/10.1128/JCM.02193-16  PMID: 28122870 
  16. Van Der Pol B, Fife K, Taylor SN, Nye MB, Chavoustie SE, Eisenberg DL, et al. Evaluation of the performance of the cobas CT/NG test for use on the cobas 6800/8800 systems for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in male and female urogenital samples. J Clin Microbiol. 2019;57(4):e01996-18.  https://doi.org/10.1128/JCM.01996-18  PMID: 30651389 
  17. Saville M, Sultana F, Malloy MJ, Velentzis LS, Caruana M, Ip ELO, et al. Clinical validation of the cobas HPV Test on the cobas 6800 system for the purpose of cervical screening. J Clin Microbiol. 2019;57(2):e01239-18.  https://doi.org/10.1128/JCM.01239-18  PMID: 30463896 
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