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Abstract

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.

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/content/10.2807/1560-7917.ES.2020.25.9.2000173
2020-03-05
2020-04-06
http://instance.metastore.ingenta.com/content/10.2807/1560-7917.ES.2020.25.9.2000173
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